(→PCR of pSB1C3 from dCas9 ST - GFP 11 clone 8 to correct mutations) |
(→Monday 19th September) |
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Line 5: | Line 5: | ||
===Visualization=== | ===Visualization=== | ||
− | ==== Glycerol stocks of clones 7, 8, 9 and 10 containing FKBP - GFP 10 in pSB1C3 ==== | + | ==== Glycerol stocks of clones 7, 8, 9 and 10 containing FKBP - GFP 10 in pSB1C3 (pPS16_018) ==== |
"By Maxence, Mahnaz Coline & Caroline" | "By Maxence, Mahnaz Coline & Caroline" | ||
The glycerol stock of the bacteria with the following plasmids were made. | The glycerol stock of the bacteria with the following plasmids were made. | ||
− | * | + | *pPS16_018 (FKBP - GFP 10) clone 7 |
− | * | + | *pPS16_018 (FKBP - GFP 10) clone 8 |
− | * | + | *pPS16_018 (FKBP - GFP 10) clone 9 |
− | * | + | *pPS16_018 (FKBP - GFP 10) clone 10 |
500µL of liquid culture and 250 µL of glycerol 60% were mixed and put at -20°C. | 500µL of liquid culture and 250 µL of glycerol 60% were mixed and put at -20°C. | ||
− | ====Plasmids extraction of clones 7, 8, 9 and 10 containing GFP 1.9 in pSB1C3==== | + | ====Plasmids extraction of clones 7, 8, 9 and 10 containing GFP 1.9 in pSB1C3 (pPS16_018)==== |
"By Maxence, Mahnaz Coline & Caroline" | "By Maxence, Mahnaz Coline & Caroline" | ||
The following plasmids were extracted using the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|"Charge Switch-Pro Plasmid Miniprep"]] kit: | The following plasmids were extracted using the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|"Charge Switch-Pro Plasmid Miniprep"]] kit: | ||
− | * | + | *pPS16_018 (FKBP - GFP 10) clone 7 |
− | * | + | *pPS16_018 (FKBP - GFP 10) clone 8 |
− | * | + | *pPS16_018 (FKBP - GFP 10) clone 9 |
− | * | + | *pPS16_018 (FKBP - GFP 10) clone 10 |
− | ====PCR of pSB1C3 | + | ====PCR of pSB1C3 with dCas9 ST - GFP 11 (pPS16_017) clone 8 to correct mutations ==== |
''By Maxence, Mahnaz, Coline & Caroline'' | ''By Maxence, Mahnaz, Coline & Caroline'' | ||
Revision as of 13:07, 19 September 2016