Difference between revisions of "Team:Freiburg/Lab"
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We want to use the Endospores of Bacillus subtilis as a carrier vessel for an prodrug activating enzyme and a nanobody to improve the targeted drug delivery system. | We want to use the Endospores of Bacillus subtilis as a carrier vessel for an prodrug activating enzyme and a nanobody to improve the targeted drug delivery system. | ||
| − | Bacillus subtilis builds these spores under distressed conditions (such as nutrition deficiency) . The spores are highly resistant to heat, UV-light and are easy to handle. | + | Bacillus subtilis builds these spores under distressed conditions (such as nutrition deficiency). The spores are highly resistant to heat, UV-light and are easy to handle. |
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| + | We modified the spores in their coat proteins, so that they are expressing the fusion proteins, the GST and nanobody, on their surface. We experimented with modifications on the gens CotZ, CgeA,CotG and Cot B, to see where we could achieve the optimal conditions. | ||
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Revision as of 23:12, 20 September 2016
Lab Summary
This year our team was working with the model organism Bacillus subtilis and Escherichia coli. Since E.coli is commonly used in many laboratories we had no problem handling it. Apparently there are only a few people who are working with bacillus subtilis, but we kindly received some help and protocols from former iGEM team Munich 2012 and support from the Bacillus Genetic Stock Center (BGSC) from Ohio who always answered our numerous questions.
We want to use the Endospores of Bacillus subtilis as a carrier vessel for an prodrug activating enzyme and a nanobody to improve the targeted drug delivery system. Bacillus subtilis builds these spores under distressed conditions (such as nutrition deficiency). The spores are highly resistant to heat, UV-light and are easy to handle.
We modified the spores in their coat proteins, so that they are expressing the fusion proteins, the GST and nanobody, on their surface. We experimented with modifications on the gens CotZ, CgeA,CotG and Cot B, to see where we could achieve the optimal conditions.
We want to use the Endospores of Bacillus subtilis as a carrier vessel for an prodrug activating enzyme and a nanobody to improve the targeted drug delivery system. Bacillus subtilis builds these spores under distressed conditions (such as nutrition deficiency). The spores are highly resistant to heat, UV-light and are easy to handle.
We modified the spores in their coat proteins, so that they are expressing the fusion proteins, the GST and nanobody, on their surface. We experimented with modifications on the gens CotZ, CgeA,CotG and Cot B, to see where we could achieve the optimal conditions.
