Difference between revisions of "Team:TEC-Costa Rica/Project/Safety"

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<a style="color: #e0e0d1;" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project">Project</a>
 
<a style="color: #e0e0d1;" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project">Project</a>
 
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<a style="color: #e0e0d1;" href="https://2016.igem.org/Team:TEC-Costa_Rica/Human_Practice">Human Practice</a>
 
<a style="color: #e0e0d1;" href="https://2016.igem.org/Team:TEC-Costa_Rica/Human_Practice">Human Practice</a>
 
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<div class="jumbotron text-center">    
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<h1>Safety</h1>
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<h1>SynBioThon 2016: rewriting the code</h1>
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</div>
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<div class="concept">
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<p style="text-align: center;">Our project is related to the detection of a human disease via a biomarker molecule found in the urine of patients with prostate cancer. Since local regulation does not allow us to work with human samples, we designed our project in a way that it can work as a proof-of-concept of a detection system and pose no risk to human health.</p>
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</div>
 
</div>
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<h2>Lab work</h2>
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<div class="concept">
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<p>During our work in the lab, we followed a series of rules to ascertain that no risks were undertaken.</p>
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<p style="text-align: center;">SynBioThon 2016 (SBT16) is a Synthetic Biology tournament, in which teams of students work for a weekend to create a genetic device that can solve some kind of problem.</p>
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<div id="safety" class="container-fluid text-center">
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      <span class="glyphicon glyphicon-heart logo-small"></span>
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      <p>Use of nitrile gloves</p>
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    <div style="width: 25%" class="col-sm-1">
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      <p>Use of safety goggles</p>
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    <div style="width: 25%" class="col-sm-1">
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      <span class="glyphicon glyphicon-leaf logo-small"></span>
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      <p>Management of bacteria in biosafety cabinet</p>
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</div>
 
</div>
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<br><br>
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<h2>Reach</h2>
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<div class="row slideanim">
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<p>For SBT16, we had diverse talks in different classes and universities, trying to reach the most diverse participants. In our college (TEC), we spoke with people of different majors (Biotechnology Engineering, Material Science, Agricultural Engineering, and others). We also went to two other public universities: University of Costa Rica (UCR), where we spoke with people of Biology, Microbiology and Pharmacy, and the National University (UNA), where wetalked to students of Biology. We also gave talks in private universities such as UCIMED (Medical School), LEAD University (Business Management), and others.</p>
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    <div style="width: 25%" class="col-sm-1">
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<p><br>Prior to the event, we had preparatory workshops with students so they could get a grasp of genetics, molecular biology, and bioinformatics if they weren’t familiar with them. We had three workshops at UCR, one at UNA, and four at TEC. We had obout 80 students overall in a full-day session. We had a theoric section where we explained the basics of genetics and molecular biology, and then an activity to test the concepts, and after lunch a demonstrative session of bioinformatics, centered in databases and benchling, which was followed by work.</p>
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      <span class="glyphicon glyphicon-certificate logo-small"></span>
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<div class="container marketing">
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      <p>Usage of volatile chemicals in fume hood</p>
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      <p>Cleaning and Sterilization of the Lab</p>
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      <h2>Projects</h2>
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      <p style="text-align: center;"><a style="color: black;" class="btn btn-default synbiothon-details" href="https://2016.igem.org/Team:TEC-Costa_Rica/Human_Practice/Synbiothon/Projects" role="button">View details &raquo;</a></p>
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      <p>Correct disposal of bio-hazardous residues</p>
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    <div class="col-lg-4">
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    <div style="width: 25%" class="col-sm-1">
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      <h2>Mentors and Judges</h2>
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      <p>Separate areas for work with bacteria, DNA and electrophoresis</p>
+
      <p style="text-align: center;"><a style="color: black;" class="btn btn-default synbiothon-details" href="https://2016.igem.org/Team:TEC-Costa_Rica/Human_Practice/Synbiothon/Mentors_Judges" role="button">View details &raquo;</a></p>
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    <div class="col-lg-4">
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      <h2>Evaluation</h2>
 +
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 +
      <p style="text-align: center;"><a style="color: black;" class="btn btn-default synbiothon-details" href="https://2016.igem.org/Team:TEC-Costa_Rica/Human_Practice/Synbiothon/Evaluation" role="button">View details &raquo;</a></p>
 +
    </div><!-- /.col-lg-4 -->
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</div>
 
</div>
 +
<div class="row">
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<h2>The winners</h2>
 +
<div class="col-sm-4">
 +
<div class="container">
 +
  <br>
 +
  <div id="myCarousel" class="carousel slide" data-ride="carousel">
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<h2>Project safety</h2>
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<p>In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.</p>
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        <img src="img_chania.jpg" alt="Chania" width="460" height="345">
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        <div class="carousel-caption">
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          <h3>Chania</h3>
 +
          <p>The atmosphere in Chania has a touch of Florence and Venice.</p>
 +
        </div>
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      </div>
 +
 
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      <div class="item">
 +
        <img src="img_chania2.jpg" alt="Chania" width="460" height="345">
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        <div class="carousel-caption">
 +
          <h3>Chania</h3>
 +
          <p>The atmosphere in Chania has a touch of Florence and Venice.</p>
 +
        </div>
 +
      </div>
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          <h3>Flowers</h3>
 +
          <p>Beatiful flowers in Kolymbari, Crete.</p>
 +
        </div>
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      </div>
 +
 
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      <div class="item">
 +
        <img src="img_flower2.jpg" alt="Flower" width="460" height="345">
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        <div class="carousel-caption">
 +
          <h3>Flowers</h3>
 +
          <p>Beatiful flowers in Kolymbari, Crete.</p>
 +
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 +
      </div>
 +
 
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 +
 
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 +
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 +
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      <span class="glyphicon glyphicon-chevron-right" aria-hidden="true"></span>
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</div>
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</div>
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<div class="col-sm-8">
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Revision as of 23:56, 23 September 2016

SynBioThon 2016: rewriting the code

SynBioThon 2016 (SBT16) is a Synthetic Biology tournament, in which teams of students work for a weekend to create a genetic device that can solve some kind of problem.

Reach

For SBT16, we had diverse talks in different classes and universities, trying to reach the most diverse participants. In our college (TEC), we spoke with people of different majors (Biotechnology Engineering, Material Science, Agricultural Engineering, and others). We also went to two other public universities: University of Costa Rica (UCR), where we spoke with people of Biology, Microbiology and Pharmacy, and the National University (UNA), where wetalked to students of Biology. We also gave talks in private universities such as UCIMED (Medical School), LEAD University (Business Management), and others.


Prior to the event, we had preparatory workshops with students so they could get a grasp of genetics, molecular biology, and bioinformatics if they weren’t familiar with them. We had three workshops at UCR, one at UNA, and four at TEC. We had obout 80 students overall in a full-day session. We had a theoric section where we explained the basics of genetics and molecular biology, and then an activity to test the concepts, and after lunch a demonstrative session of bioinformatics, centered in databases and benchling, which was followed by work.

Generic placeholder image

Projects

Donec sed odio dui. Etiam porta sem malesuada magna mollis euismod. Nullam id dolor id nibh ultricies vehicula ut id elit. Morbi leo risus, porta ac consectetur ac, vestibulum at eros. Praesent commodo cursus magna.

View details »

Generic placeholder image

Mentors and Judges

Duis mollis, est non commodo luctus, nisi erat porttitor ligula, eget lacinia odio sem nec elit. Cras mattis consectetur purus sit amet fermentum. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh.

View details »

Generic placeholder image

Evaluation

Donec sed odio dui. Cras justo odio, dapibus ac facilisis in, egestas eget quam. Vestibulum id ligula porta felis euismod semper. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus.

View details »

The winners


ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

iGEM TEC Costa Rica

Tecnológico de Costa Rica
Biotechnology Investigation Center
Cartago, Costa Rica
prostaltec@gmail.com

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