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| | {{Paris_Pasteur_Microbiology}} | | {{Paris_Pasteur_Microbiology}} |
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| − | <div class="lightbox" id="exp12"> | + | <div class="lightbox" id="exp1"> |
| | <figure> | | <figure> |
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| | </figcaption> | | </figcaption> |
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| − | </div>
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| − | <div class="lightbox" id="exp11">
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| − | <figure>
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| − | <a href="#" class="closemsg"></a>
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| − | <figcaption>
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| − | <p>
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| − | <U> Aim:</U> Measure the quantity of plasmid using a Nanodrop (Thermofisher) before sending for sequencing (inserts B1 and C2)</br></br>
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| − | <U> Protocol:</U> follow in this link </br></br>
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| − | <U>What we did in the lab:</U></br>
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| − | <U>Materials:</U></br>
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| − | • Nanodrop (Thermofisher)</br>
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| − | • Elution buffer from QIAGEN kit</br>
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| − | • Microbiology equipment (Follow this link)</br></br>
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| − | <U>Method:</U></br>
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| − | Analyze absorbance at 260nm</br>
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| − | 1. Clean the Nanodrop with water</br>
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| − | 2. Make the blank with 1 µl of elution buffer</br>
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| − | 3. Put 1ul of your sample on the Nanodrop</br>
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| − | 4. Make the measure and clean the Nanodrop between each measure</br></br>
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| − | <U>Results:</U></br>
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| − | <table>
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| − | <thead>
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| − | <tr>
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| − | <th>λ= 260 nm</th>
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| − | <th>B1(1)</th>
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| − | <th>B1(2)</th>
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| − | <th>C2>th>
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| − | </tr>
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| − | </thead>
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| − | <tbody>
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| − | <tr>
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| − | <td><strong><p>A<sub>DNA</sub></p></strong></td>
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| − | <td>0.725</td>
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| − | <td>0.741</td>
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| − | <td>0.761</td>
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| − | </tr>
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| − | <tr>
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| − | <tr>
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| − | <td><strong>C final</strong></td>
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| − | <td>36.3 ng/µl</td>
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| − | <td>37.0 ng/µl</td>
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| − | <td>38.0 ngµl</td>
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| − | </tr>
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| − |
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| − | </tbody>
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| − | </table>
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| − | <U>Absorbance</U>
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| − | </br></br></br>
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| − | 5. Preparation of the samples for sequencing: put 15 µl of DNA and add 2 µl of fitted primers</br>
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| − | 6. Send the samples for sequencing</br>
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| − | </p>
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| − | </figcaption>
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| − | </figure>
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| | </div> | | </div> |
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