Difference between revisions of "Team:Peking/Notebook"

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<h3 style="text-align:center"> Protocols</h3>
 
<h3 style="text-align:center"> Protocols</h3>
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<div class="four columns">
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:SDS-Pages"><img src="https://static.igem.org/mediawiki/2016/d/d4/T--Peking--image_protocol_SDS_Pages.png"></a>
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<p style="text-align:center">SDS-Pages</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:ligation"><img src="https://static.igem.org/mediawiki/2016/2/2f/T--Peking--image_protocol_ligation.png"></a>
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<p style="text-align:center">Ligation</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:temperature_gradient_experiment"><img src="https://static.igem.org/mediawiki/2016/c/c1/T--Peking--image_protocol_temperature_gradient_experiment.png"></a>
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<p style="text-align:center">Temperature Gradient Experiment</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:PCR"><img src="https://static.igem.org/mediawiki/2016/0/09/T--Peking--image_protocol_PCR.png"></a>
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<p style="text-align:center">PCR</p>
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</div>
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<div class="four columns">
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:purification_of_recombinant_proteins"><img src="https://static.igem.org/mediawiki/2016/4/48/T--Peking--image_protocol_purification_of_recombinant_proteins.png"></a>
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<p style="text-align:center">Purification of Recombinant Proteins</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:transformation"><img src="https://static.igem.org/mediawiki/2016/5/52/T--Peking--image_protocol_transformation.png"></a>
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<p style="text-align:center">Transformation</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:testing_adsorption_capacity_of_protein"><img src="https://static.igem.org/mediawiki/2016/8/85/T--Peking--image_protocol_testing_adsorption_capacity_of_protein.png"></a>
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<p style="text-align:center">Testing Adsorption Capacity of Protein</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:AGE"><img src="https://2016.igem.org/File:T--Peking--image_protocol_AGE.png"></a>
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<p style="text-align:center">Agarose Gel Electrophoresis</p>
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</div>
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<div class="four columns">
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:biotin-associated_Experiment"><img src="https://static.igem.org/mediawiki/2016/b/b4/T--Peking--image_protocol_biotin-associated_Experiment.png"></a>
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<p style="text-align:center">Biotin-associated Experiment</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:Gel_Extraction"><img src="https://static.igem.org/mediawiki/2016/0/03/T--Peking--image_protocol_Gel_Extraction.png"></a>
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<p style="text-align:center">Gel Extraction</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:Western_Blot"><img src="https://static.igem.org/mediawiki/2016/1/10/T--Peking--image_protocol_Western_Blot.png"></a>
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<p style="text-align:center">Western Blot</p>
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<a href="https://2016.igem.org/Team:Peking/Notebook/Protocol:pH_Gradient_Experiment"><img src="https://static.igem.org/mediawiki/2016/9/97/T--Peking--image_protocol_pH_Gradient_Experiment.png"></a>
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<p style="text-align:center">pH Gradient Experiment</p>
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</div>
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Revision as of 07:17, 1 October 2016

Standard

Notebook.

In this page, you can view how we worked on our project.

Diary

Week 1 (6/26/2016-7/02/2016)

  • We transformed BioBricks from the distribution:
    • BBa_J33201 (arsR)
    • BBa_K516030 (mRFP1 with RBS and terminator)
    • Colonies were used to inoculate overnight cultures.
    • Plasmids were isolated using a miniprep kit.

Week 2 (4/20/2015-4/26/2015)

...

Week 3 (4/20/2015-4/26/2015)

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Week 4 (4/20/2015-4/26/2015)

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Week 5 (4/20/2015-4/26/2015)

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Week 6 (4/20/2015-4/26/2015)

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Week 7 (4/20/2015-4/26/2015)

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Week 8 (4/20/2015-4/26/2015)

...

Week 9 (4/20/2015-4/26/2015)

...

Protocols

SDS-Pages

Ligation

Temperature Gradient Experiment

PCR

Purification of Recombinant Proteins

Transformation

Testing Adsorption Capacity of Protein

Agarose Gel Electrophoresis

Biotin-associated Experiment

Gel Extraction

Western Blot

pH Gradient Experiment