Difference between revisions of "Team:Peking/Notebook"

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<p style="text-align:center">Gel Extraction</p>
 
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<p style="text-align:center">Western Blot</p>
 
<p style="text-align:center">Western Blot</p>

Revision as of 07:23, 1 October 2016

Standard

Notebook.

In this page, you can view how we worked on our project.

Dairy

Week 1 (6/26/2016-7/02/2016)

  • We transformed BioBricks from the distribution:
    • BBa_J33201 (arsR)
    • BBa_K516030 (mRFP1 with RBS and terminator)
    • Colonies were used to inoculate overnight cultures.
    • Plasmids were isolated using a miniprep kit.

Week 2 (4/20/2015-4/26/2015)

...

Week 3 (4/20/2015-4/26/2015)

...

Week 4 (4/20/2015-4/26/2015)

...

Week 5 (4/20/2015-4/26/2015)

...

Week 6 (4/20/2015-4/26/2015)

...

Week 7 (4/20/2015-4/26/2015)

...

Week 8 (4/20/2015-4/26/2015)

...

Week 9 (4/20/2015-4/26/2015)

...

Protocols

Gel Extraction

Ligation

Temperature Gradient Experiment

PCR

Purification of Recombinant Proteins

Transformation

Testing Adsorption Capacity of Protein

Agarose Gel Electrophoresis

Biotin-associated Experiment

SDS-Page

Western Blot

pH Gradient Experiment