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We want to use the endospores of <i>Bacillus subtilis</i> as a carrier vessel for a prodrug activating enzyme and a nanobody to improve the targeted drug delivery system. | We want to use the endospores of <i>Bacillus subtilis</i> as a carrier vessel for a prodrug activating enzyme and a nanobody to improve the targeted drug delivery system. | ||
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Revision as of 21:40, 3 October 2016
Lab Summary
We want to use the endospores of Bacillus subtilis as a carrier vessel for a prodrug activating enzyme and a nanobody to improve the targeted drug delivery system.
Bacillus subtilis builds these spores under distressed conditions (such as nutrition deficiency). The spores are highly resistant to heat, UV-light and are easy to handle.
We modified the spores in their coat proteins so that they are expressing the fusion proteins, the Glutathione S-Transferase (GST) and nanobody, on their surface. We experimented with modifications on the genes CotZ, CgeA,CotG and CotB, to see where we could achieve the optimal conditions.
