Difference between revisions of "Team:Paris Saclay/Notebook/October/10"
(→BioBrick K2039000 and K2039001 characterization) |
(→Protein electrophoresis of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and PhB1040 strain 594) |
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A protein electrophoresis of the extracted protein was done using the [[Team:Paris_Saclay/Experiments#Protein_electrophoresis_using_the_ThermoFisher_Mini_Gel_Tank.C2.AE_device|protein electrophoresis protocol]]. For that purpose, the gel cassette Bolt 4-12% Bis-Tris Plus 10W was used and the power was set for 1 hour at 65V. | A protein electrophoresis of the extracted protein was done using the [[Team:Paris_Saclay/Experiments#Protein_electrophoresis_using_the_ThermoFisher_Mini_Gel_Tank.C2.AE_device|protein electrophoresis protocol]]. For that purpose, the gel cassette Bolt 4-12% Bis-Tris Plus 10W was used and the power was set for 1 hour at 65V. | ||
| + | ====transfert of protein extracted from FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and PhB1040 strain 594==== | ||
| + | ''By Maxence'' | ||
| + | |||
| + | The proteins on the electrophoresis gel were transfered on blotting membrane using the [[Team:Paris_Saclay/Experiments#Protein_transfert_using_the_ThermoFisher_iBlot_2_Dry_Blotting_System.C2.AE|protein transfert protocol]]. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 14:05, 12 October 2016
