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| | <h1>Tuesday, June 21<sup>st</sup></h3> | | <h1>Tuesday, June 21<sup>st</sup></h3> |
| | <h2>Agenda:</h2> | | <h2>Agenda:</h2> |
| − | | + | <ul> |
| | + | <li>Grow up DH5α</li> |
| | + | <li>Review <em>S. aureus</em> Cas9 sequence</li> |
| | + | </ul> |
| | + | <h2>Results: </h2> |
| | + | <p>All cells save 2 lines from the previous day did not grow. The most likely reason for the transformation failure is a typo on the iGEM website. The procedure said to resuspend the DNA in 100 uL of water, but the instructions most likely meant 10 uL. We believe the excess DNA over-stressed the competent cells. </p> |
| | + | <h2>Tasks:</h2> |
| | + | <div class="row"> |
| | + | <div class="col-sm-2"> |
| | + | <p>Shu & Sara</p> |
| | + | </div> |
| | + | <div class="col-sm-10"> |
| | + | <ul> |
| | + | <li>Grew 5 more 5 mL LB + DH5a E. coli tubes</li> |
| | + | <li>Incubated overnight<br> |
| | + | </li> |
| | + | </ul> |
| | + | </div> |
| | + | </div> |
| | + | <div class="row"> |
| | + | <div class="col-sm-2"> |
| | + | <p>Michelle</p> |
| | + | </div> |
| | + | <div class="col-sm-10"> |
| | + | <ul> |
| | + | <li>Wrote iGEM preliminary description for the website</li> |
| | + | </ul> |
| | + | </div> |
| | + | </div> |
| | + | <div class="row"> |
| | + | <div class="col-sm-2"> |
| | + | <p>Tyler & Sara</p> |
| | + | </div> |
| | + | <div class="col-sm-10"> |
| | + | <ul> |
| | + | <li>Found the sequence encoding Cas9 in <em>Staphylococcus</em> <em>aureus</em>.</li> |
| | + | </ul> |
| | + | </div> |
| | + | </div> |
| | <footer id="nav"> | | <footer id="nav"> |
| | <div class="row"> | | <div class="row"> |
