Project description

Chemotaxis

Chemotaxis is the movement of an organism in response to an external chemical stimulus. Many single-cell and multicellular organisms use chemotaxis to navigate their immediate environment.

The E. coli chemotaxis system is considered a model system that illustrates some of the core principles of chemotactic movement (1). It allows the cell to sense and quickly respond to nearby nutrients – attractants, and dangerous chemicals – repellents.

Chemoreceptors – A bacterial sensor system

Bacterial chemotaxis is mediated by chemoreceptors. The purpose of these membrane proteins is to bind a certain chemoeffector, and transduce the signal to the downstream proteins.

The specificity of a chemoreceptor is determined by its ligand binding domain – the transmembrane region of the receptor. We have chosen this domain as the focus of our project.

S.Tar – In control of chemotaxis

Project Super Tar - S.Tar in short, is designed to be a novel platform for controlled chemotaxis. The base of our project is the E. coli Tar chemoreceptor or more specifically, its ligand binding domain (LBD). We show that by mutating the native Tar LBD (2) or by interchanging it with that of other receptors (3), the E. coli chemotaxis system can be programmed to respond to completely new ligands.

To demonstrate the potential of our system, we performed two experiments: 1. We replaced the Tar LBD with that of the PctA (link to the chimeras page) chemoreceptor and demonstrated (link to PctA results) a chemotactic response to its repellent – PEC, a substance which the native Tar cannot recognize.
2. We used computational biology to redesign the native Tar receptor (link to Rosetta page) to bind Histamine instead of its original ligand. We demonstrated a chemotactic response from the mutated receptor (link to histamine results).
Using S.Tar, scientists will be able to control the movement of bacteria and direct them towards or away from a target material. This system can have vast applications in bioremediation and, as we show in our project, detection.

FlashLab – A S.Tar detector

As a further application of our system we designed an easy-to-use detection system which utilizes the high sensitivity of the chemotactic response. FlashLab (link to flashlab page) is a simple and cheap platform for the detection of any chemoeffector, using S.Tar bacteria.