Difference between revisions of "Team:Technion Israel"
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| − | <p> | + | <p>We, the Technion iGEM team of 2016, are developing <b>FlashLab</b> - a user-friendly chip for rapid detection of various substances such as hormones and heavy metals. The detection relies on the E. coli chemotaxis system, which allows bacteria to move away from or towards target materials. In comparison to existing biosensors, this system is expected to combine simplicity, speed and universality - offering the ability to detect diverse materials using the same method without the need for expensive lab equipment. </p> |
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| + | <p>The idea is to use a bacterial chemoreceptor and exchange its ligand-binding domain (LBD) with a small library of various LBDs. This library will be based on (1) natural bacterial chemoreceptors and (2) novel LBDs; designed using the RosettaCommons software suite. Moreover, to expand the repertoire of LBDs we plan to use an innovative approach to introduce self-splicing inteins as triggers for chemotactic activity. </p> | ||
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| + | <p>To observe the bacteria, which co-express both the redesigned chemoreceptor and a color protein, several different designs of microchips will be tested. The bacteria will be confined to a microchannel and chemotactic movement, induced by the target material, will lead to the formation of clusters that are visible to the naked eye. Given the simplicity of the design, the chip is expected to work much like a home pregnancy test – just insert a sample and wait for the answer! </p> | ||
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Revision as of 11:12, 27 June 2016
Project Description
We, the Technion iGEM team of 2016, are developing FlashLab - a user-friendly chip for rapid detection of various substances such as hormones and heavy metals. The detection relies on the E. coli chemotaxis system, which allows bacteria to move away from or towards target materials. In comparison to existing biosensors, this system is expected to combine simplicity, speed and universality - offering the ability to detect diverse materials using the same method without the need for expensive lab equipment.
The idea is to use a bacterial chemoreceptor and exchange its ligand-binding domain (LBD) with a small library of various LBDs. This library will be based on (1) natural bacterial chemoreceptors and (2) novel LBDs; designed using the RosettaCommons software suite. Moreover, to expand the repertoire of LBDs we plan to use an innovative approach to introduce self-splicing inteins as triggers for chemotactic activity.
To observe the bacteria, which co-express both the redesigned chemoreceptor and a color protein, several different designs of microchips will be tested. The bacteria will be confined to a microchannel and chemotactic movement, induced by the target material, will lead to the formation of clusters that are visible to the naked eye. Given the simplicity of the design, the chip is expected to work much like a home pregnancy test – just insert a sample and wait for the answer!
Before you start:
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2016 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples:
Uploading pictures and files
You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to Team:YourOfficialTeamName/NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
