Difference between revisions of "Team:IngenuityLab Canada/Attributions"

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<h1>Project Attributions</h1>
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On behalf of Ingenuity Lab’s 2016 iGEM Team, we thank our Director and Principle Investigator: Dr. Carlo Montemagno. Our Secondary Principle Investigator: Dr. Gane Ka-Shu Wong. Especially for their never-ending support and guidance.
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</p><p>
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All lab work including primer and Biobrick construct design, PCRs, minipreps, cloning, transformations, cyanobacteria growth, photosystem II protein purification, PSII proteoliposome reconstitution, oxygen evolution assays, DNA origami reactions, gel electrophoresis, SDS-PAGE, nanodropping, blotting were performed by the student members of the iGEM IngenuityLab_Canada only.
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</p><p>
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We are forever grateful to our advisors/instructors for offering their extensive scientific expertise, both experimentally and in computational modelling: Sibel Cetinel (DNA Origami), Hiofan Hoi (DNA Origami), Hosna Jabbari (Computational approach to nucleic acid origami), Prashanthi Kovur (Teslaphoresis), and Kyle Minor (<i>Synechocystis sp</i>. PCC 6803 and Photosystem II Purification).
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</p><p>
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Acquisition of the His-tagged CP47 to Photosystem II complex mutant Synechocystis sp. PCC 6803 was obtained from Kyle Minor who obtained it from Dr. Terry M. Bricker of Louisiana State University.
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</p><p>
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Special thanks to Kyle Minor (Ingenuity Lab) for teaching us the culturing techniques for growing Synechocystis sp. PCC 6803. In addition to teaching us how to purify photosystem II protein. 
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</p><p>
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Critical insight into genetic engineering in cyanobacteria that led us to confidently design our Biobrick constructs: Professor George Owttrim, Faculty of Science, Department of Biological Sciences, University of Alberta, Edmonton, Canada.
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</p><p>
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Professor Michael Ellison (Biochemistry, University of Alberta) for testing our octahedron DNA nanoparticle as a proof of concept for an updated iGEM Registry submission standard. Specifically for DNA Origami parts submission.
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</p><p>
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A huge thank you to Ingenuity Lab’s Laboratory Support Staff for receiving our orders, maintaining a clean laboratory space, and general organization of lab space: Jeanine Koshelek, Matthew Christensen, JoJo (Hang Zhou), and Diana Pham
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</p><p>
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A huge thank you to Ingenuity Lab’s Administration Team for processing our orders, administration guidance and support: Susan Kuzmak, Melissa Germain, Jeffrey Germain, and Shaoli
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</p><p>
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DNA Synthesis of BioBrick parts, DNA oligonucleotides was purchased from IDT DNA Inc. thanks to their generous offer to all iGEM 2016 teams.
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</p><p>
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Transmission Electron Microscopy Facility: Dr. Xuejun Sun and Mrs. Priscilla Gao of the Cross Cancer Institute, Edmonton, Alberta, Canada.
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</p><p>
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Nanodrop Usage: Biochemistry Facility, maintained by Angela Brigley, located in National Institute for Nanotechnology, Edmonton, Canada
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</p><p>
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DNA Sequencing: pay-per-use service offered by the Molecular Biology Service Unit (MBSU), University of Alberta, Edmonton, Canada
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</p><p>
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Policies and Practices (Intellectual Property Rights in Canada): TEC Edmonton and Martin Ferguson Pell
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</p><p>
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Policies and Practices (Intellectual Property Rights in China): Sichuan University iGEM team (SCU-China)
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</p><p>
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Funding and Professional Development Workshops: MindFuel and geekStarter (AITF Alberta)
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</p><p>
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Website Design: World News Media Limited. London, United Kingdom
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</p><p>
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DNA Origami and Photosystem II Animations: Danny Kinsman
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">Attributions bronze criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
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<h5> Why is this page needed? </h5>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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<h5> What should this page have?</h5>
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<li>General Support</li>
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<li>Project support and advice</li>
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<li>Fundraising help and advice</li>
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<li>Lab support</li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
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<li>Wiki support</li>
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<li>Presentation coaching</li>
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<li>Human Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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<h5> Can we base our project on a previous one? </h5>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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<h5>Inspiration</h5>
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<p>Take a look at what other teams have done:</p>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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<h5>Team training and Project start</h5>
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
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<li>When did you start this course?</li>
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<li>Are the syllabus and course materials freely available online?</li>
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<li>When did you start your brainstorming?</li>
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<li>When did you start in the lab?</li>
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<li>When did you start working on  your project?</li>
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Revision as of 17:31, 13 October 2016

Ingenuity Lab - dNANO

 

Project Attributions

On behalf of Ingenuity Lab’s 2016 iGEM Team, we thank our Director and Principle Investigator: Dr. Carlo Montemagno. Our Secondary Principle Investigator: Dr. Gane Ka-Shu Wong. Especially for their never-ending support and guidance.

All lab work including primer and Biobrick construct design, PCRs, minipreps, cloning, transformations, cyanobacteria growth, photosystem II protein purification, PSII proteoliposome reconstitution, oxygen evolution assays, DNA origami reactions, gel electrophoresis, SDS-PAGE, nanodropping, blotting were performed by the student members of the iGEM IngenuityLab_Canada only.

We are forever grateful to our advisors/instructors for offering their extensive scientific expertise, both experimentally and in computational modelling: Sibel Cetinel (DNA Origami), Hiofan Hoi (DNA Origami), Hosna Jabbari (Computational approach to nucleic acid origami), Prashanthi Kovur (Teslaphoresis), and Kyle Minor (Synechocystis sp. PCC 6803 and Photosystem II Purification).

Acquisition of the His-tagged CP47 to Photosystem II complex mutant Synechocystis sp. PCC 6803 was obtained from Kyle Minor who obtained it from Dr. Terry M. Bricker of Louisiana State University.

Special thanks to Kyle Minor (Ingenuity Lab) for teaching us the culturing techniques for growing Synechocystis sp. PCC 6803. In addition to teaching us how to purify photosystem II protein.

Critical insight into genetic engineering in cyanobacteria that led us to confidently design our Biobrick constructs: Professor George Owttrim, Faculty of Science, Department of Biological Sciences, University of Alberta, Edmonton, Canada.

Professor Michael Ellison (Biochemistry, University of Alberta) for testing our octahedron DNA nanoparticle as a proof of concept for an updated iGEM Registry submission standard. Specifically for DNA Origami parts submission.

A huge thank you to Ingenuity Lab’s Laboratory Support Staff for receiving our orders, maintaining a clean laboratory space, and general organization of lab space: Jeanine Koshelek, Matthew Christensen, JoJo (Hang Zhou), and Diana Pham

A huge thank you to Ingenuity Lab’s Administration Team for processing our orders, administration guidance and support: Susan Kuzmak, Melissa Germain, Jeffrey Germain, and Shaoli

DNA Synthesis of BioBrick parts, DNA oligonucleotides was purchased from IDT DNA Inc. thanks to their generous offer to all iGEM 2016 teams.

Transmission Electron Microscopy Facility: Dr. Xuejun Sun and Mrs. Priscilla Gao of the Cross Cancer Institute, Edmonton, Alberta, Canada.

Nanodrop Usage: Biochemistry Facility, maintained by Angela Brigley, located in National Institute for Nanotechnology, Edmonton, Canada

DNA Sequencing: pay-per-use service offered by the Molecular Biology Service Unit (MBSU), University of Alberta, Edmonton, Canada

Policies and Practices (Intellectual Property Rights in Canada): TEC Edmonton and Martin Ferguson Pell

Policies and Practices (Intellectual Property Rights in China): Sichuan University iGEM team (SCU-China)

Funding and Professional Development Workshops: MindFuel and geekStarter (AITF Alberta)

Website Design: World News Media Limited. London, United Kingdom

DNA Origami and Photosystem II Animations: Danny Kinsman