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Revision as of 17:45, 13 October 2016
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Collaborations
Protocol videos
Our team decided to create protocol videos in order to help young scientists in manipulation. During our experiments, we filmed 4 steps of cloning: PCR, digestion, ligation and transformation. We asked other teams to translate protocols in their mother tongue. We obtained protocol videos in English with the Imperial College London team, in Spanish thanks to the TEC-Monterrey team, in Dutch with Leiden team, in Chinese with BIT-China team, in Portuguese thanks to EEL-USP team and in French with the collaboration of the Bordeaux team.
We wrote the entire script and we added some guidance in red (minutes for each paragraphs for instance). Teams had to translate and record the script. Now, we possess a lot of protocols explained in six different languages, accessible to everybody on Youtube and on our wiki.
Tutorials
Click on the flag to see the video
Digestion
This video presents the protocol to do enzymatic digestion of a DNA sample. This technique is useful for usual clonings.
Imperial College of London team
Tuto digestion
Ligation
When a DNA fragment is digested with digestion enzymes, it is possible to ligate its extremities with another digested fragment, if the extremities are cohesives. This method can be used to add an insert in a vector or a backbone.
tuto ligation
PCR
PCR, or Polymerase Chain Reaction, is a technique that amplify a DNA sequence by using primers and DNA polymerase. Throughout this video, you will see how to perform a PCR. A theoretical explanation of the way how PCR works ends the video.
c
Transformation
A transformation is a method that allows to insert a plasmid in a E. coli bacteria. Several method to transform a bacteria exist, but we chose to use a thermal shock method for bacteria to become permeable.
Growth study of B. subtilis and P. fluorescens
In exchange for recording the script in English, we studied the optimal growth of Bacillus subtilis and Pseudomonas fluorescens for the Imperial London College team. First, we did an experimental plan to observe if pH is in interaction with temperature. Finally, the conclusion was that pH and temperature were two independent parameters. We ran the experiments again, changing only one parameter.
Results
Optimum Temperature (°C) |
Optimum pH |
Doubling time (minutes) |
|
Bacillus subtilis (WT 168) |
34-37 |
8 | 41 |
Pseudomonas fluorescens (SBW25) |
<29 |
7,5 | <80 |
Optimum temperatures and pH and the doubling times are summarized in the table for our two strains.
3D conception
Bordeaux
Receipt of plasmids
We want to thank the 2012 Munich team for sending us 4 different shuttle plasmids. One of these, pBs0k-p, was very useful because we used it for cloning in E.coli and Bacillus subtilis.
Mailing of kit
2016 Evry team asked us to send a Ludox solution that was in our iGEM Kit. They stored their own solution at -20°C so it was not usable anymore. But it was important for them to participate to the InterLab project so we helped them.
Protocol translation
2016 METU HS Ankara team wanted to create a database, which consists of different language protocols. They asked us to translate their protocol in French.
Surveys
We filled in different surveys all along the year. Among them, surveys for:
- Bdx
- IONIS
- Evry
- Valencia
- TecCEM Collegiate
- Purdue
- UBGM
Website by Team iGEM Toulouse 2016
