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Revision as of 07:31, 14 October 2016
Parts
Overview of Parts
Our team submitted a total of 6 parts to the iGEM registry. The parts that were submitted were all components to AHL quorum sensing systems. The parts are all RFC21 compatible with other parts possessing other compatibilities. There are 5 basic parts that are part of the part collection and 1 composite part. The senders for the Aub, Bja, Bra, Cer, and Sin systems were submitted as part of the inductance test with the F2620 part, and compose a submitted part collection. The 1 composite part is the modular vector for the inducer.
Quorum Sensing-F2620 Inductions
The part Bba_F2620 (found here), designed by Barry Canton and Anna Labno from MIT, is a device designed to output PoPs when LuxR is activated. This was used to test interactions between inducers from other quorum sensing systems. The Aub, Bja, Bra, Cer, and Sin systems stem from various organisms and their inducers were submitted as parts to the registry with the purpose of completing this induction test.
N-Acyl Homoserine Lactones
AHL quorum sensing has a myriad of different systems. A total of 10 systems were investigated in this project
| AHL System | Bacteria of Origin | AHL Name | 3D-Model |
|---|---|---|---|
| Aub | Unknown | N-(2-oxooxolan-3-yl)dodecanamide |  |
| Bja | Bradyrhizobium japonicum | 3-methyl-N-[(3S)-2-oxooxolan-3-yl]butanamide |  |
| Bra | Paraburkholderia kururiensis | (3S)-3-[(2-oxo-3-phenylpropyl)amino]oxolan-2-one |  |
| Cer | Rhodobacter sphaeroides | (Z)-3-hydroxy-N-[(3S)-2-oxooxolan-3-yl]tetradec-7-enamide |  |
| Esa | Erwinia stewartii | 3-oxo-N-[(3S)-2-oxooxolan-3-yl]hexanamide |  |
| Las | Pseudomonas aeruginosa | 3-oxo-N-(2-oxooxolan-3-yl)dodecanamide |  |
| Lux | Vibrio fischeri | 3-oxo-N-(2-oxooxolan-3-yl)hexanamide |  |
| Rhl | Rhizobium leguminosarum | N-(2-oxooxolan-3-yl)butanamide | |
| Rpa | Rhodopseudomonas palustris | (S)-(−)α-amino-γ-butyrolactone |  |
| Sin | Sinorhizobium meliloti | N-[(3S)-2-oxooxolan-3-yl]octanamide* |  |
*Sin system produces 6 different variants of AHL. The 3D structures of all the Sin compounds can be found here.
All systems were investigated in an inductions investigation. The part BBa_F2620 was used to induce production in the Lux AHL system and test induction in any other AHL systems. Should induction occur, then possible interference between systems are conceivable, which may have implications towards any use of that system. The resulting part collection allows direct comparison in AHL induction between multiple systems.
Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <groupparts> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.
Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
