Difference between revisions of "Team:Chalmers Gothenburg/Safety"

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  <title>Chalmers Gothenburg iGEM 2016</title>
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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    <div class="header-text">NOTEBOOK</div>
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    <div class="header-subtitle">Safety</div>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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            <a href="#lab_safety" class="active">Lab safety</a>
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            <a href="#risk_declaration">Risk declaration</a>
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            <a href="#MSDS">MSDS</a>
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            <a href="#Risk_assessments">Risk assessments</a>
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            <p class="text" id="lab_safety">Before starting the wetlab part of the project all team members
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            had to take part in a safety lecture held by the lab manager at the Systems and  
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            synthetic biology department. During this lecture the eam learned how to handle
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            chemicals and waste in the lab, we also had a lab tour where the safety equipment
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            were introduced. During the lab tour all the responsible research engineers were
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            present and gave us introductions to all of the different lab equipments they are
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            responsible for.</p>
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            <p class="text">As a safety measurement, each team member had to read the
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            materials safety data sheet for the materials used and the KLARA risk assessments
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            of the machines handled during the summer. Each member then had to sign the risk
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            declaration to testify that one had taken part of this information and gain access
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            to the laboratory.</p>
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            <h3 class="sidetitle" id="risk_declaration">Risk declaration</h3>
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            <ul>
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                <li class="list">SOP risk declaration guidelines</li>
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                <li class="list">Risk Declaration 2016</li>
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            </ul>
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            <h3 class="sidetitle" id="MSDS">MSDS - Material Safety Data Sheet</h3>
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            <ul>
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                <li class="list">Chloramphenicol</li>
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                <li class="list">Ampicillin</li>
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                <li class="list">G418 disulfate salt colution</li>
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                <li class="list">GelRed Nucleic acid stain</li>
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                <li class="list">Kanamycin sulfate</li>
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                <li class="list">Spectinomycin hydrochloride</li>
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            </ul>
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            <h3 class="sidetitle" id="Risk_assessments">KLARA Risk assessments</h3>
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            <ul>
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                <li class="list">Dishwasher</li>
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                <li class="list">-80°C freezer</li>
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                <li class="list">Bench-top autoclave</li>
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                <li class="list">Centrifugal vacuum vaporator</li>
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                <li class="list">Centrifuge (Avanti J-20 XP)</li>
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                <li class="list">Centrifuge (Sigma 4K15)</li>
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                <li class="list">Gel electrophoresis with GelRed</li>
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                <li class="list">Gel electrophoresis</li>
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                <li class="list">Heat block</li>
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                <li class="list">Rotary shakers, Incubators</li>
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                <li class="list">Small centrifuges</li>
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                <li class="list">LAF bench</li>
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                <li class="list">Thermal cycler (PCR)</li>
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            </ul>
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        </div>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h5>Safe Lab Work</h5>
 
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h5>Safe Shipment</h5>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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Revision as of 12:19, 14 October 2016

Chalmers Gothenburg iGEM 2016

NOTEBOOK
Safety

Before starting the wetlab part of the project all team members had to take part in a safety lecture held by the lab manager at the Systems and synthetic biology department. During this lecture the eam learned how to handle chemicals and waste in the lab, we also had a lab tour where the safety equipment were introduced. During the lab tour all the responsible research engineers were present and gave us introductions to all of the different lab equipments they are responsible for.

As a safety measurement, each team member had to read the materials safety data sheet for the materials used and the KLARA risk assessments of the machines handled during the summer. Each member then had to sign the risk declaration to testify that one had taken part of this information and gain access to the laboratory.

Risk declaration

  • SOP risk declaration guidelines
  • Risk Declaration 2016

MSDS - Material Safety Data Sheet

  • Chloramphenicol
  • Ampicillin
  • G418 disulfate salt colution
  • GelRed Nucleic acid stain
  • Kanamycin sulfate
  • Spectinomycin hydrochloride

KLARA Risk assessments

  • Dishwasher
  • -80°C freezer
  • Bench-top autoclave
  • Centrifugal vacuum vaporator
  • Centrifuge (Avanti J-20 XP)
  • Centrifuge (Sigma 4K15)
  • Gel electrophoresis with GelRed
  • Gel electrophoresis
  • Heat block
  • Rotary shakers, Incubators
  • Small centrifuges
  • LAF bench
  • Thermal cycler (PCR)