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Line 3: =Existing parts used for the project=
=Existing parts used for the project=
− In order to construct or own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:
+ In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:
Revision as of 16:00, 14 October 2016
Parts
Existing parts used for the project In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:
Name
Type
Description
Designer
Length (bp)
[http://parts.igem.org/Part:BBa_J23119/ BBa_J23119]
Basic
Constitutive promoter family member
iGEM2006_Berkeley
35
[http://parts.igem.org/Part:BBa_B0030/ BBa_B0030]
Basic
RBS.1 (strong) - modified from R. Weiss
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003
15
[http://parts.igem.org/Part:BBa_B0015/ BBa_B0015]
Basic
Double terminator (B0010-B0012) (rrnB T1 - T7Te)
Reshma Shetty, 2003
129
Parts designed for the project In order to achieve our project; we designed several basic and composite parts:
Name
Type
Description
Length (bp)
[http://parts.igem.org/Part:BBa_K2039003/ BBa_K2039003]
Basic
Coding sequences of proteins FKBP and GFP10
528
[http://parts.igem.org/Part:BBa_K2039004/ BBa_K2039004]
Basic
Coding sequences of proteins FRB* and GFP11
498
[http://parts.igem.org/Part:BBa_K2039000/ BBa_K2039000]
Composite
Part expressing the dimerization protein FKBP and GFP10
731
[http://parts.igem.org/Part:BBa_K2039001/ BBa_K2039001]
Composite
Part expressing the dimerization protein FRB* and GFP11
701
[http://parts.igem.org/Part:BBa_K2039002/ BBa_K2039002]
Composite
Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)
1440
