Difference between revisions of "Team:Alverno CA/Proof"

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<p><h3>Proof of Concept</h3></p>
 
<p><h3>Proof of Concept</h3></p>
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<p>We have constructed a set of RFP/GFP reporters (BBa_K2145000-BBa_K2145003) with a large spacer between the two reporter genes. The reporter set includes all of the combinations of orientations of the two genes, and will demonstrate orientation-dependent expression interference between genes on the same plasmid. This set of constructs demonstrates the need for new techniques for insulating the components of multi-gene circuits on the same piece of DNA.</p>
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<p>We constructed a set of RFP/GFP reporters (BBa_K2145000-BBa_K2145003) with a large spacer between the two reporter genes. The reporter set includes all of the combinations of orientations of the two genes, and will demonstrate orientation-dependent expression interference between genes on the same plasmid. This set of constructs demonstrates the need for new techniques for insulating the components of multi-gene circuits on the same piece of DNA.</p>

Revision as of 20:00, 15 October 2016

Alverno iGEM 2016

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Proof of Concept

We constructed a set of RFP/GFP reporters (BBa_K2145000-BBa_K2145003) with a large spacer between the two reporter genes. The reporter set includes all of the combinations of orientations of the two genes, and will demonstrate orientation-dependent expression interference between genes on the same plasmid. This set of constructs demonstrates the need for new techniques for insulating the components of multi-gene circuits on the same piece of DNA.