Line 22: | Line 22: | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
<!---------------------------Main content section of page-------------------------------> | <!---------------------------Main content section of page-------------------------------> |
Revision as of 21:23, 16 October 2016
![](https://static.igem.org/mediawiki/2016/9/93/T--Virginia--Parts_header.png)
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
![](https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png)
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence.
![](https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png)
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.