Difference between revisions of "Team:Alverno CA/Collaborations"
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<figure width=700><img src="https://static.igem.org/mediawiki/2016/e/e9/T--Alverno_CA--image%281%29.png" alt="iGEM" style="width:60%; height:60%"> | <figure width=700><img src="https://static.igem.org/mediawiki/2016/e/e9/T--Alverno_CA--image%281%29.png" alt="iGEM" style="width:60%; height:60%"> | ||
| − | <figcaption width=700>This chart represents the fluorescence of the plasmids (each one <br> labeled according to | + | <figcaption width=700>This chart represents the fluorescence of the plasmids (each one <br> labeled according to their RBS part number as well as their iGEM <br> part number) measured in Arbitrary Fluorescence Units in TX-TL. </figcaption> |
</figure></p> | </figure></p> | ||
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Revision as of 23:28, 16 October 2016
Collaborations
TX-TL is a cell-free, in vitro, transcription/translation system that allows for inexpensive and rapid prototyping of biological circuits. This is desirable, as the usual method of prototyping and debugging circuits requires DNA parts to be cloned into cells, which can be time-consuming and laborious. With TX-TL, once all the DNA parts have been obtained, the circuit can be tested immediately, and so several circuit iterations can be tested in the time it takes to successfully clone even one circuit iteration into cells. Caltech generously loaned us a plate reader to enable us to use this method.
We were able to successfully perform TX-TL for a series of William and Mary parts.
We offered TX-TL William and Mary’s Team’s plasmids in order to see how their results would vary with this different construct. William and Mary’s Team gave us suggestions and also offered us some plasmid and backbone which is iGEM standard plasmid.
Below is a graph of our results
labeled according to their RBS part number as well as their iGEM
part number) measured in Arbitrary Fluorescence Units in TX-TL.
