Difference between revisions of "Team:Ionis Paris/Parts"

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                             <h1 id="back_to_the_top">Parts and Characterization</h1>
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                             <h1 id="back_to_the_top">Favorite </h1>
 
                              
 
                              
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  <h2 class="secHd">Context</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>For our projet, we focused on air pollution. We had an in-depth reflexion about the existing measurement techniques, and found out that, among all the existing devices, none of them was satisfying. During our benchmark, we also realized that most of these devices were placed on fixed measurement station, and that they were by consequent unable to give precise results on a smaller scale, which are more and more needed du to the ever-increasing health concerns. Learn all the details of why we believe our project can be a real breakthrough in terms of air pollution measurement.</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Design" ><font color="DeepPink">Here</font></a>
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<div class="section_title">
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                            <h2 class="secHd">Our Core Project</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>We started to work on a biosensor, a modified cell able to respond to a given signal. The goal of our cell would be to respond to the detection of a specific pollutant by emitting bioluminescence. We used the XylR protein, known to bind to molecules such as the toluene and benzene (two important pollutants), and once bound to activate the Pu promoter. Activation of this promoter would then trigger luciferase synthesis, allowing bioluminescence. Once our biosensor plasmid assembled, we used E. coli bacteria as a chassis organism.</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Biology"><font color="DeepPink">The Biology behind Quantifly</font></a>
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      </div>
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        <!-- ====ABOUT US==== -->
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<!-- ====ABOUT US==== -->
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<div class="section_title">
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                            <h2 class="secHd">Measurement</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. Thus, we tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Measurement" ><font color="DeepPink">Learn more about our work with CelloCad</font></a>
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                    <div class="col-xs-12 col-sm-6">
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<div class="section_title">
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                            <h2 class="secHd">BioBricks</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our:</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Parts"><font color="DeepPink">Parts & Characterization</font></a>
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</br><a href="https://2016.igem.org/Team:Ionis_Paris/Proof"><font color="DeepPink">Proof of Concept</font></a>
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                        </div>
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                    </div>
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      </div>
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<p> A mettre dans cette page: le tableau des parts, ainsi que le lien vers le registry. Le zip des fichiers de séquençages. Mentionner résultats de Cello et biosenseur et linker vers les parties correspondantes </p>
 
<p> A mettre dans cette page: le tableau des parts, ainsi que le lien vers le registry. Le zip des fichiers de séquençages. Mentionner résultats de Cello et biosenseur et linker vers les parties correspondantes </p>
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Revision as of 14:48, 17 October 2016

Parts and characterization

Context

For our projet, we focused on air pollution. We had an in-depth reflexion about the existing measurement techniques, and found out that, among all the existing devices, none of them was satisfying. During our benchmark, we also realized that most of these devices were placed on fixed measurement station, and that they were by consequent unable to give precise results on a smaller scale, which are more and more needed du to the ever-increasing health concerns. Learn all the details of why we believe our project can be a real breakthrough in terms of air pollution measurement.

Here

Our Core Project

We started to work on a biosensor, a modified cell able to respond to a given signal. The goal of our cell would be to respond to the detection of a specific pollutant by emitting bioluminescence. We used the XylR protein, known to bind to molecules such as the toluene and benzene (two important pollutants), and once bound to activate the Pu promoter. Activation of this promoter would then trigger luciferase synthesis, allowing bioluminescence. Once our biosensor plasmid assembled, we used E. coli bacteria as a chassis organism.

The Biology behind Quantifly

Measurement

As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. Thus, we tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.

Learn more about our work with CelloCad

BioBricks

We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our:

Parts & Characterization
Proof of Concept

A mettre dans cette page: le tableau des parts, ainsi que le lien vers le registry. Le zip des fichiers de séquençages. Mentionner résultats de Cello et biosenseur et linker vers les parties correspondantes