Difference between revisions of "Team:Paris Saclay/Notebook/June/29"
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====Culture of clones with gBlocks ==== | ====Culture of clones with gBlocks ==== | ||
''By Lea and Marion'' | ''By Lea and Marion'' | ||
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| + | We got 6 clones of each construction in 3,5ml LB (50 µg/ml Amp). Incubation overnight at 37°C, 180rpm. | ||
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| + | ===BioBrick characterization=== | ||
| + | ====BL21 electro-competent cells preparation==== | ||
| + | ''By Charlene'' | ||
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| + | We added 200µL of BL21 pre-culture in 15mL of LB. It incubated 4h at 37°C. When abs600nm=0,68 cells have been centrifuged 10 minutes at 4000rpm. We did 2 washes with addition of 10mL glycerol (10%) followed by 10 minutes of centrifugation at 4000rpm. We putted cells in 200 µL glycerol. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 15:26, 4 July 2016
