Difference between revisions of "Team:Paris Saclay/Notebook/July/4"
(Created page with "{{Team:Paris_Saclay") |
|||
| Line 1: | Line 1: | ||
| − | {{Team:Paris_Saclay | + | {{Team:Paris_Saclay/notebook_header}} |
| + | =Monday 4<sup>th</sup> July= | ||
| + | ==Lab work== | ||
| + | |||
| + | ===Visualization=== | ||
| + | ====Digestion of plasmids==== | ||
| + | ''By Mathilde and Alice'' | ||
| + | |||
| + | The following plasmids were digested again with EcoR1 and HindIII: | ||
| + | *1.1 (from the 6 clones that were selected on 29/06/16) | ||
| + | *2.1 (from the 6 clones that were selected on 29/06/16) | ||
| + | *3.1 (from the 6 clones that were selected on 29/06/16) | ||
| + | *3.2 (from the 6 clones that were selected on 29/06/16) | ||
| + | |||
| + | The same digestion protocol as on {{Font color||yellow|???}} was followed increasing the DNA quantity (10µL DNA, 7µL water, 2µL Red buffer, 0.5µL EcoR1, 0.5µL HindIII). Digestion products were migrated on agarose gel. | ||
| + | The DNA concentration was still insufficient meaning the extraction step was not efficient. New extraction will be performed on clones got after the transformation. | ||
| + | |||
| + | |||
| + | ===Bringing DNA closer=== | ||
| + | ====Plasmids extraction==== | ||
| + | ''By Naiane and Laetitia'' | ||
| + | |||
| + | Plasmids from the following strains were extracted with the kit "Charge Switch-Pro Plasmid Miniprep": | ||
| + | *DS-NMCas | ||
| + | *DS-SPCasN | ||
| + | *DS-ST1 | ||
| + | *DS-TDCasN | ||
| + | Then plasmids were digested with AvrII increasing the DNA quantity (10µL DNA, 1µL AvrII, 2µL Tango buffer, 7µL water). Digestion products were migrated (5µL digestion, 5µL water, 2µL {{Font color||Yellow|bleu de charge}} | ||
Revision as of 16:37, 4 July 2016
