Difference between revisions of "Team:Ionis Paris/Parts"

Line 157: Line 157:
 
                         </div>
 
                         </div>
 
                         <div class="about_text">
 
                         <div class="about_text">
 +
 +
<table>
 +
 +
<tr>
 +
<td> </td>
 +
<td> Part Name </td>
 +
<td> Nickname </td>
 +
<td> Type </td>
 +
<td> Designer </td>
 +
<td> Lengh</td>
 +
 +
</tr>
 +
 +
<tr>
 +
<td> Bba_K2023005
 +
</td>
 +
<td> XylR  </td>
 +
<td> BB2 </td>
 +
<td>Coding
 +
</td>
 +
<td> C.Soucies </td>
 +
<td> 1704 bp </td>
 +
</tr>
 +
 +
</table>
  
  
 
  <p>
 
  <p>
−
We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our:</p>
+
</p>
  
 
<a href="https://2016.igem.org/Team:Ionis_Paris/Parts"><font color="DeepPink">Parts & Characterization</font></a>
 
<a href="https://2016.igem.org/Team:Ionis_Paris/Parts"><font color="DeepPink">Parts & Characterization</font></a>
Line 171: Line 196:
 
         <!-- ====ABOUT US==== -->
 
         <!-- ====ABOUT US==== -->
 
      
 
      
 +
 +
 +
 +
 +
 +
 +
<!-- ====ABOUT US==== -->
 +
 +
            <div class="container">
 +
                <div class="row">
 +
                    <div class="col-xs-12 col-sm-6">
 +
<div class="section_title">
 +
 +
 +
                           
 +
                            <h2 class="secHd">Biosensor parts list</h2>
 +
                           
 +
                        </div>
 +
                        <div class="about_text">
 +
                         
 +
<table>
 +
 +
<tr>
 +
<td> </td>
 +
<td> Part Name </td>
 +
<td> Nickname </td>
 +
<td> Type </td>
 +
<td> Designer </td>
 +
<td> Lengh</td>
 +
 +
</tr>
 +
 +
<tr>
 +
<td> Term-Pu-RBS-Gluc-term </td>
 +
<td>  </td>
 +
<td> BB3 </td>
 +
<td> Composite
 +
 +
</td>
 +
<td> B.Piot </td>
 +
<td> 1330 bp </td>
 +
</tr>
 +
 +
</table>
 +
 +
 +
 +
  <p>
 +
 +
 +
 +
</p>
 +
 +
<a href="https://2016.igem.org/Team:Ionis_Paris/Measurement" ><font color="DeepPink">Learn more about our work with CelloCad</font></a>
 +
                        </div>
 +
                    </div>
 +
 +
                 
 +
                        </div>
 +
                    </div>
 +
      </div>
 +
         
 +
 +
        <!-- ====ABOUT US==== -->
 +
   
 +
 +
 +
 +
  
  

Revision as of 20:09, 17 October 2016

Favorite Part: our biosensor

Part Name Nickname Type Designer Lengh
Bba_K2023001 Biosensor device BB123 Measurement C.Chenebault, C.Soucies, B.Piot 3156 bp

The biosensor is our favorite part because it is the heart of our project and because it took us a lot time to create. Like it is already explain in the Biobrick Design section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.

Biosensor parts list

Part Name Nickname Type Designer Lengh
Bba_K2023004 Pr-RBS BB1 Composite C.Chenebault 448 bp

Learn more about our work with CelloCad

B

Part Name Nickname Type Designer Lengh
Bba_K2023005 XylR BB2 Coding C.Soucies 1704 bp

Parts & Characterization
Proof of Concept

Biosensor parts list

Part Name Nickname Type Designer Lengh
Term-Pu-RBS-Gluc-term BB3 Composite B.Piot 1330 bp

Learn more about our work with CelloCad

Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria.

Learn more about our Hardware

We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly.

Learn more about the Entrepreunarial aspects of our project