Difference between revisions of "Team:Ionis Paris/Parts"
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Revision as of 20:49, 17 October 2016
The biosensor is our favorite part because it is the heart of our project and because it took us a lot time to create. Like it is already explain in the Biobrick Design section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.
Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria. We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly.Favorite Part: our biosensor
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023001
Biosensor device
BB123
Measurement
C.Chenebault, C.Soucies, B.Piot
3156 bp
Biosensor parts list
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023004
Pr-RBS
BB1
Composite
C.Chenebault
448 bp
enlever titre
A enlever
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023006
Term-Pu-RBS-Gluc-term
BB3
Composite
B.Piot
1330 bp
Biosensor parts list
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023002
XylR coding device
BBA
Regulatory
C.Chenebault
2261 bp
enlever titre
Part Name
Nickname
Type
Designer
Lengh
Bba_K2023003
Gluc coding device with Pu
BBB
Reporter
C.Soucies
1028 bp
