Difference between revisions of "Team:Ionis Paris/Parts"

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                             <h2 class="secHd">Favorite Part: our biosensor</h2>
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                             <h2 class="secHd">Our biosensor</h2>
 
                              
 
                              
 
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<p>
 
<p>
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The biosensor is our favorite part because it is the heart of our project and because it took us a lot time to create. Like it is already explain in the <a href="https://2016.igem.org/Team:Ionis_Paris/Biobrick_Design">Biobrick Design</a> section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.
+
The biosensor is the heart of our project and because it took us a lot time to create. Like it is already explain in the <a href="https://2016.igem.org/Team:Ionis_Paris/Biobrick_Design">Biobrick Design</a> section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.
  
 
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                             <h2 class="secHd">Biosensor parts list</h2>
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                             <h2 class="secHd">Favorite Parts</h2>
 
                              
 
                              
 
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                             <h2 class="secHd">Biosensor parts list</h2>
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                             <h2 class="secHd">virer titre</h2>
 
                              
 
                              
 
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<div class="section_title">
  
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                            <h2 class="secHd">Gaussia derived parts</h2>
 
                              
 
                              
 
                         </div>
 
                         </div>
 
                         <div class="about_text">
 
                         <div class="about_text">
−
                            <p>Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria.</p>
+
                         
 +
<table>
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Hardware" ><font color="DeepPink">Learn more about our Hardware</font></a>
+
<tr>
−
                        </div>
+
<td> </td>
−
                    </div>
+
<td> Part Name </td>  
 +
<td> Nickname </td>  
 +
<td> Type </td>  
 +
<td> Designer </td>  
 +
<td> Lengh</td>  
  
−
                    <div class="col-xs-12 col-sm-6">
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</tr>
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<div class="section_title">
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                        </div>
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                        <div class="about_text">
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                            <p>We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly.</p>
+
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Entrepreneurship"><font color="DeepPink">Learn more about the Entrepreunarial aspects of our project</font></a>
+
<tr>
 +
<td> <a href="http://parts.igem.org/Part:Bba_K2023007" ><font color="DeepPink">Bba_K2023007</font></a></td>
 +
<td> Gluc coding device with Pr  </td>
 +
<td> G1 </td>
 +
<td> device
 +
</td>
 +
<td> B.Piot </td>
 +
<td> 1120 bp </td>
 +
</tr>
 +
 +
</table>
  
−
                        </div>
 
−
                    </div>
 
  
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        <!-- ====ABOUT US==== -->
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            <div class="container">
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</p>
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<div class="section_title">
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−
                            <h2 class="secHd">Biosensor parts list</h2>
 
−
                           
 
−
                        </div>
 
−
                        <div class="about_text">
 
−
                            <p>As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. Thus, we tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.</p>
 
−
 
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Measurement" ><font color="DeepPink">Learn more about our work with CelloCad</font></a>
 
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
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<div class="section_title">
 
                              
 
                              
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                           <h2 class="secHd">  B     </h2>
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                           <h2 class="secHd">  enlever titre     </h2>
 
                      
 
                      
 
                         </div>
 
                         </div>
 
                         <div class="about_text">
 
                         <div class="about_text">
 +
 +
<table>
 +
 +
<tr>
 +
<td> </td>
 +
<td> Part Name </td>
 +
<td> Nickname </td>
 +
<td> Type </td>
 +
<td> Designer </td>
 +
<td> Lengh</td>
 +
 +
</tr>
 +
 +
<tr>
 +
<td> <a href="Bba_K2023008"><font color="DeepPink">Bba_K2023008</font></a>
 +
</td>
 +
<td> Gluc-term </td>
 +
<td> G2 </td>
 +
<td>Composite
 +
</td>
 +
<td> C.Soucies </td>
 +
<td> 663 bp </td>
 +
</tr>
 +
 +
</table>
  
  
 
  <p>
 
  <p>
−
We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our:</p>
+
</p>
 +
 
 +
 
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Parts"><font color="DeepPink">Parts & Characterization</font></a>
 
−
</br><a href="https://2016.igem.org/Team:Ionis_Paris/Proof"><font color="DeepPink">Proof of Concept</font></a>
 
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
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<div class="section_title">
  
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                            <h2 class="secHd">A enlever</h2>
 
                              
 
                              
 
                         </div>
 
                         </div>
 
                         <div class="about_text">
 
                         <div class="about_text">
−
                            <p>Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria.</p>
+
                         
 +
<table>
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Hardware" ><font color="DeepPink">Learn more about our Hardware</font></a>
+
<tr>
−
                        </div>
+
<td> </td>
−
                    </div>
+
<td> Part Name </td>  
 +
<td> Nickname </td>  
 +
<td> Type </td>  
 +
<td> Designer </td>  
 +
<td> Lengh</td>  
  
−
                    <div class="col-xs-12 col-sm-6">
+
</tr>
−
<div class="section_title">
+
−
                           
+
−
                           
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−
                            <h2 class="secHd"> </h2>
+
−
                   
+
−
                        </div>
+
−
                        <div class="about_text">
+
−
                            <p>We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly.</p>
+
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Entrepreneurship"><font color="DeepPink">Learn more about the Entrepreunarial aspects of our project</font></a>
+
<tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2023006" ><font color="DeepPink">BBa_K2023006</font></a>
 +
  </td>
 +
<td>Term-Pu-RBS-Gluc-term  </td>
 +
<td> BB3 </td>
 +
<td> Composite
  
−
                        </div>
+
</td>  
−
                    </div>
+
<td> B.Piot </td>  
−
 
+
<td> 1330 bp </td>  
−
           
+
</tr>
−
 
+
−
                        </div>
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−
                    </div>
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      </div>
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      </div>
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      </div>
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      </div>
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        <!-- ====ABOUT US==== -->
+
 
   
 
   
 +
</table>
  
  
  
 +
  <p>
  
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 +
</p>
  
−
                           
 
−
                            <h2 class="secHd">Biosensor parts list</h2>
 
−
                           
 
−
                        </div>
 
−
                        <div class="about_text">
 
−
                            <p>As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. Thus, we tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.</p>
 
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Measurement" ><font color="DeepPink">Learn more about our work with CelloCad</font></a>
 
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
  
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                        </div>
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                        <div class="about_text">
+
−
 
+
−
 
+
−
<p>
+
−
We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our:</p>
+
−
 
+
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Parts"><font color="DeepPink">Parts & Characterization</font></a>
+
−
</br><a href="https://2016.igem.org/Team:Ionis_Paris/Proof"><font color="DeepPink">Proof of Concept</font></a>
+
 
                         </div>
 
                         </div>
 
                     </div>
 
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<div class="section_title">
 
  
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              <h2 class="secHd"> </h2>
 
−
                           
 
−
             
 
−
                           
 
−
                        </div>
 
−
                        <div class="about_text">
 
−
                            <p>Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria.</p>
 
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Hardware" ><font color="DeepPink">Learn more about our Hardware</font></a>
 
−
                        </div>
 
−
                    </div>
 
  
−
                    <div class="col-xs-12 col-sm-6">
 
−
<div class="section_title">
 
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                            <h2 class="secHd"> </h2>
 
−
                   
 
−
                        </div>
 
−
                        <div class="about_text">
 
−
                            <p>We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly.</p>
 
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Entrepreneurship"><font color="DeepPink">Learn more about the Entrepreunarial aspects of our project</font></a>
 
  
−
                        </div>
 
−
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−
           
 
  
−
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        <!-- ====ABOUT US==== -->
 
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−
<!-- ====ABOUT US==== -->
 
  
−
            <div class="container">
 
−
                <div class="row">
 
−
                    <div class="col-xs-12 col-sm-6">
 
−
<div class="section_title">
 
  
  
−
                           
 
−
                            <h2 class="secHd">Biosensor parts list</h2>
 
−
                           
 
−
                        </div>
 
−
                        <div class="about_text">
 
−
                            <p>As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. Thus, we tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.</p>
 
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Measurement" ><font color="DeepPink">Learn more about our work with CelloCad</font></a>
 
−
                        </div>
 
−
                    </div>
 
  
−
                    <div class="col-xs-12 col-sm-6">
 
−
<div class="section_title">
 
−
                           
 
−
                          <h2 class="secHd">  B      </h2>
 
−
                   
 
−
                        </div>
 
−
                        <div class="about_text">
 
  
  
−
<p>
 
−
We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our:</p>
 
  
−
<a href="https://2016.igem.org/Team:Ionis_Paris/Parts"><font color="DeepPink">Parts & Characterization</font></a>
 
−
</br><a href="https://2016.igem.org/Team:Ionis_Paris/Proof"><font color="DeepPink">Proof of Concept</font></a>
 
−
                        </div>
 
−
                    </div>
 
−
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−
         
 
  
−
        <!-- ====ABOUT US==== -->
 
−
   
 
  
  
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                            <p>Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria.</p>
 
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                            <p>We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly.</p>
 
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<a href="https://2016.igem.org/Team:Ionis_Paris/Entrepreneurship"><font color="DeepPink">Learn more about the Entrepreunarial aspects of our project</font></a>
 
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Revision as of 22:20, 17 October 2016

Our biosensor

Part Name Nickname Type Designer Lengh
BBa_K2023001 Biosensor device BB123 Measurement C.Chenebault, C.Soucies, B.Piot 3156 bp

The biosensor is the heart of our project and because it took us a lot time to create. Like it is already explain in the Biobrick Design section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.

Favorite Parts

Part Name Nickname Type Designer Lengh
BBa_K2023004 Pr-RBS BB1 Composite C.Chenebault 448 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023005 XylR BB2 Coding C.Soucies 1704 bp

A enlever

Part Name Nickname Type Designer Lengh
BBa_K2023006 Term-Pu-RBS-Gluc-term BB3 Composite B.Piot 1330 bp

virer titre

Part Name Nickname Type Designer Lengh
BBa_K2023002 XylR coding device BBA Regulatory C.Chenebault 2261 bp

enlever titre

Part Name Nickname Type Designer Lengh
BBa_K2023003 Gluc coding device with Pu BBB Reporter C.Soucies 1028 bp

Parts for CelloCad

Part Name Nickname Type Designer Lengh
Bba_K2023014 GFP coding device with Pr C2 Device V.Plait 1314

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023015 XylR coding device-GFP coding device with Pu C3 Measurement C.Soucies 3391 bp

A virer

Part Name Nickname Type Designer Lengh
BBa_Bba_K2023017 Pu-RBS C4 Composite C.Chenebault 358 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023016 GFP coding device with Pu C45 Reporter C.Soucies 1224 bp

Gaussia derived parts

Part Name Nickname Type Designer Lengh
Bba_K2023007 Gluc coding device with Pr G1 device B.Piot 1120 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023008 Gluc-term G2 Composite C.Soucies 663 bp

A enlever

Part Name Nickname Type Designer Lengh
BBa_K2023006 Term-Pu-RBS-Gluc-term BB3 Composite B.Piot 1330 bp