Difference between revisions of "Team:Ionis Paris/Parts"

Line 506: Line 506:
  
 
<tr>
 
<tr>
−
<td> <a href="http://parts.igem.org/Part:BBa_Bba_K2023017" ><font color="DeepPink">BBa_Bba_K2023017</font></a></td>
+
<td> <a href="http://parts.igem.org/Part:Bba_K2023017" ><font color="DeepPink">Bba_K2023017</font></a></td>
 
<td> Pu-RBS  </td>
 
<td> Pu-RBS  </td>
 
<td>C4 </td>  
 
<td>C4 </td>  
Line 739: Line 739:
  
  
 +
<!-- ====ABOUT US==== -->
  
 +
            <div class="container">
 +
                <div class="row">
 +
                    <div class="col-xs-12 col-sm-6">
 +
<div class="section_title">
 +
 +
 +
                           
 +
                            <h2 class="secHd">XylR derived parts</h2>
 +
                           
 +
                        </div>
 +
                        <div class="about_text">
 +
                         
 +
<table>
 +
 +
<tr>
 +
<td> </td>
 +
<td> Part Name </td>
 +
<td> Nickname </td>
 +
<td> Type </td>
 +
<td> Designer </td>
 +
<td> Lengh</td>
 +
 +
</tr>
 +
 +
<tr>
 +
<td> <a href="http://parts.igem.org/Part:Bba_K2023010" ><font color="DeepPink">Bba_K2023010</font></a></td>
 +
<td> XylR with His tag
 +
  </td>
 +
<td> X2 </td>
 +
<td> Coding
 +
</td>
 +
<td> C.Soucies</td>
 +
<td> 1667 </td>
 +
</tr>
 +
 +
</table>
 +
 +
 +
 +
  <p>
 +
 +
 +
 +
</p>
 +
 +
                        </div>
 +
                    </div>
 +
 +
                    <div class="col-xs-12 col-sm-6">
 +
<div class="section_title">
 +
                           
 +
                          <h2 class="secHd">  enlever titre      </h2>
 +
                   
 +
                        </div>
 +
                        <div class="about_text">
 +
 +
<table>
 +
 +
<tr>
 +
<td> </td>
 +
<td> Part Name </td>
 +
<td> Nickname </td>
 +
<td> Type </td>
 +
<td> Designer </td>
 +
<td> Lengh</td>
 +
 +
</tr>
 +
 +
<tr>
 +
<td> <a href="Bba_K2023011"><font color="DeepPink"> Bba_K2023011</font></a>
 +
</td>
 +
<td> XylR-His tag coding device  </td>
 +
<td> X3 </td>
 +
<td>Regulatory
 +
 +
</td>
 +
<td> C.Soucies </td>
 +
<td> 2319 bp </td>
 +
</tr>
 +
 +
</table>
 +
 +
 +
<p>
 +
</p>
 +
 +
 +
 +
                        </div>
 +
                    </div>
 +
      </div>
 +
         
 +
 +
        <!-- ====ABOUT US==== -->
 +
   
 +
 +
 +
 +
 +
<!-- ====ABOUT US==== -->
 +
 +
            <div class="container">
 +
                <div class="row">
 +
                    <div class="col-xs-12 col-sm-6">
 +
<div class="section_title">
 +
 +
 +
                           
 +
                            <h2 class="secHd">A virer</h2>
 +
                           
 +
                        </div>
 +
                        <div class="about_text">
 +
                         
 +
<table>
 +
 +
<tr>
 +
<td> </td>
 +
<td> Part Name </td>
 +
<td> Nickname </td>
 +
<td> Type </td>
 +
<td> Designer </td>
 +
<td> Lengh</td>
 +
 +
</tr>
 +
 +
<tr>
 +
<td> <a href="http://parts.igem.org/Part:Bba_K2023012" ><font color="DeepPink">Bba_K2023012</font></a></td>
 +
<td> BB1- XylR-His tag    </td>
 +
<td>BB12His </td>
 +
<td> Composite
 +
</td>
 +
<td> C.Chenebault </td>
 +
<td> 1864 bp </td>
 +
</tr>
 +
 +
</table>
 +
 +
 +
 +
  <p>
 +
 +
 +
 +
</p>
 +
 +
                        </div>
 +
                    </div>
 +
 +
                    <div class="col-xs-12 col-sm-6">
 +
<div class="section_title">
 +
                           
 +
                          <h2 class="secHd">  enlever titre      </h2>
 +
                   
 +
                        </div>
 +
                        <div class="about_text">
 +
 +
<table>
 +
 +
<tr>
 +
<td> </td>
 +
<td> Part Name </td>
 +
<td> Nickname </td>
 +
<td> Type </td>
 +
<td> Designer </td>
 +
<td> Lengh</td>
 +
 +
</tr>
 +
 +
<tr>
 +
<td> <a href="Bba_K2023013"><font color="DeepPink">Bba_K2023013</font></a>
 +
</td>
 +
<td> XylR coding device with mRFP    </td>
 +
<td> X1 </td>
 +
<td>Regulatory
 +
 +
 +
</td>
 +
<td>B.Piot</td>
 +
<td> 3023 bp </td>
 +
</tr>
 +
 +
</table>
 +
 +
 +
<p>
 +
</p>
 +
 +
 +
 +
                        </div>
 +
                    </div>
 +
      </div>
 +
         
 +
 +
        <!-- ====ABOUT US==== -->
 +
   
  
  

Revision as of 23:05, 17 October 2016

Our biosensor

Part Name Nickname Type Designer Lengh
BBa_K2023001 Biosensor device BB123 Measurement C.Chenebault, C.Soucies, B.Piot 3156 bp

The biosensor is the heart of our project and because it took us a lot time to create. Like it is already explain in the Biobrick Design section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.

Favorite Parts

Part Name Nickname Type Designer Lengh
BBa_K2023004 Pr-RBS BB1 Composite C.Chenebault 448 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023005 XylR BB2 Coding C.Soucies 1704 bp

A enlever

Part Name Nickname Type Designer Lengh
BBa_K2023006 Term-Pu-RBS-Gluc-term BB3 Composite B.Piot 1330 bp

virer titre

Part Name Nickname Type Designer Lengh
BBa_K2023002 XylR coding device BBA Regulatory C.Chenebault 2261 bp

enlever titre

Part Name Nickname Type Designer Lengh
BBa_K2023003 Gluc coding device with Pu BBB Reporter C.Soucies 1028 bp

Parts for CelloCad

Part Name Nickname Type Designer Lengh
Bba_K2023014 GFP coding device with Pr C2 Device V.Plait 1314

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023015 XylR coding device-GFP coding device with Pu C3 Measurement C.Soucies 3391 bp

A virer

Part Name Nickname Type Designer Lengh
Bba_K2023017 Pu-RBS C4 Composite C.Chenebault 358 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023016 GFP coding device with Pu C45 Reporter C.Soucies 1224 bp

Gaussia derived parts

Part Name Nickname Type Designer Lengh
Bba_K2023007 Gluc coding device with Pr G1 device B.Piot 1120 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023008 Gluc-term G2 Composite C.Soucies 663 bp

A enlever

Part Name Nickname Type Designer Lengh
Bba_K2023009 Gluc optimized G3 Coding B.Piot 561 bp

XylR derived parts

Part Name Nickname Type Designer Lengh
Bba_K2023010 XylR with His tag X2 Coding C.Soucies 1667

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023011 XylR-His tag coding device X3 Regulatory C.Soucies 2319 bp

A virer

Part Name Nickname Type Designer Lengh
Bba_K2023012 BB1- XylR-His tag BB12His Composite C.Chenebault 1864 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023013 XylR coding device with mRFP X1 Regulatory B.Piot 3023 bp