Difference between revisions of "Team:Austin UTexas/Parts"
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<h4>Blue Chromoprotein</h4> | <h4>Blue Chromoprotein</h4> | ||
| − | <p><a href="http://parts.igem.org/Part:BBa_K2097001">K2097001</a> is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, | + | <p><a href="http://parts.igem.org/Part:BBa_K2097001">K2097001</a> This is a composite part made up of <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> (blue chromoprotein) and <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> is made up of a promoter, <a href="http://partsregistry.org/Part:BBa_J23104"> BBa_J23104</a>, and an RBS, <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_B0034</a>. |
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<h4>Yellow-Green Chromoprotein</h4> | <h4>Yellow-Green Chromoprotein</h4> | ||
Revision as of 23:10, 17 October 2016
Austin_UTexas
Registry Parts
pH Sensors
CpxA-CpxR
CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in Shigella sonnei, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. The construct can be found on the iGEM registry as: K2097000, as well as on our results page here.
Composite Parts
Blue Chromoprotein
K2097001 This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B0034.
Yellow-Green Chromoprotein
K2097002 is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein)and BBa_K608006 (a medium promoter and RBS). LINKS
