Difference between revisions of "Team:Ionis Paris/Parts"

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<td> 448 bp </td>  
 
<td> 448 bp </td>  
 
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<td> <a href="Bba_K2023005"><font color="DeepPink">Bba_K2023005</font></a>
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<td> XylR  </td>
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<td> BB2 </td>
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<td>Coding
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</td>
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<td> C.Soucies </td>
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<td> 1704 bp </td>
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<td><a href="http://parts.igem.org/Part:BBa_K2023006" ><font color="DeepPink">BBa_K2023006</font></a>
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  </td>
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<td>Term-Pu-RBS-Gluc-term  </td>
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<td> BB3 </td>
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<td> Composite
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</td>
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<td> B.Piot </td>
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<td> 1330 bp </td>
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</table>
  

Revision as of 23:25, 17 October 2016

Our biosensor

Part Name Nickname Type Designer Lengh
BBa_K2023001 Biosensor device BB123 Measurement C.Chenebault, C.Soucies, B.Piot 3156 bp

The biosensor is the heart of our project and because it took us a lot time to create. Like it is already explain in the Biobrick Design section, this biobrick is composed of the Pr promoter (Bba_K2023004) which is a constitutive promoter driving the transcription of the XylR gene (Bba_K2023005) coding for the XylR protein. The XylR protein is able to bind aromatic hydrocarbons that carry a methyl group like toluene and xylene. When XylR has bind toluene for instance, it is able to regulate the Pu promoter has a transcriptional regulator. Then, Pu is activated and it allows the transcription of the bioluminescent reporter gene GLuc (Pu+GLuc: Bba_K2023003), coding for the Gaussia luciferase. When this enzyme reacts with its substrate luciferine, a substance called Coelenterazine, it emits luminescence.

Favorite Parts

Part Name Nickname Type Designer Lengh
BBa_K2023004 Pr-RBS BB1 Composite C.Chenebault 448 bp
Bba_K2023005 XylR BB2 Coding C.Soucies 1704 bp
BBa_K2023006 Term-Pu-RBS-Gluc-term BB3 Composite B.Piot 1330 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023005 XylR BB2 Coding C.Soucies 1704 bp

A enlever

Part Name Nickname Type Designer Lengh
BBa_K2023006 Term-Pu-RBS-Gluc-term BB3 Composite B.Piot 1330 bp

virer titre

Part Name Nickname Type Designer Lengh
BBa_K2023002 XylR coding device BBA Regulatory C.Chenebault 2261 bp

enlever titre

Part Name Nickname Type Designer Lengh
BBa_K2023003 Gluc coding device with Pu BBB Reporter C.Soucies 1028 bp

Parts for CelloCad

Part Name Nickname Type Designer Lengh
Bba_K2023014 GFP coding device with Pr C2 Device V.Plait 1314

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023015 XylR coding device-GFP coding device with Pu C3 Measurement C.Soucies 3391 bp

A virer

Part Name Nickname Type Designer Lengh
Bba_K2023017 Pu-RBS C4 Composite C.Chenebault 358 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023016 GFP coding device with Pu C45 Reporter C.Soucies 1224 bp

Gaussia derived parts

Part Name Nickname Type Designer Lengh
Bba_K2023007 Gluc coding device with Pr G1 device B.Piot 1120 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023008 Gluc-term G2 Composite C.Soucies 663 bp

A enlever

Part Name Nickname Type Designer Lengh
Bba_K2023009 Gluc optimized G3 Coding B.Piot 561 bp

XylR derived parts

Part Name Nickname Type Designer Lengh
Bba_K2023010 XylR with His tag X2 Coding C.Soucies 1667

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023011 XylR-His tag coding device X3 Regulatory C.Soucies 2319 bp

A virer

Part Name Nickname Type Designer Lengh
Bba_K2023012 BB1- XylR-His tag BB12His Composite C.Chenebault 1864 bp

enlever titre

Part Name Nickname Type Designer Lengh
Bba_K2023013 XylR coding device with mRFP X1 Regulatory B.Piot 3023 bp