Difference between revisions of "Team:Paris Saclay/Notebook/June/23"

(Thursday 23rd June)
Line 13: Line 13:
 
''by Caroline, Lea and Marion''
 
''by Caroline, Lea and Marion''
  
Colonies were observed in all conditions except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.  
+
Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.  
  
 
====Transformed cell cultures====
 
====Transformed cell cultures====
 
''by Lea''
 
''by Lea''
  
One colony was taken into 3,5mL LB medium + Cm at 30µg/mL for each condition (except of course for device 1).  
+
All colonies got after transformation were  One colony was taken into 3,5mL LB medium + Cm at 30µg/mL for each condition (except of course for device 1).  
  
 
{{Team:Paris_Saclay/notebook_footer}}
 
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 13:50, 6 July 2016

Thursday 23rd June

Lab work

Competent cells

by Caroline and Lea

A transformation was made with Interlab study constructions using the same protocol as 22/06/2016 but this time with our competent cells. A negative control and pSB1C3 blue plasmids (positive control) were also transformed and displayed.


Interlab study

Transformation results

by Caroline, Lea and Marion

Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.

Transformed cell cultures

by Lea

All colonies got after transformation were One colony was taken into 3,5mL LB medium + Cm at 30µg/mL for each condition (except of course for device 1).