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''by Lea''
− All colonies got after transformation were One colony was taken into 3,5mL LB medium + Cm at 30µg/mL for each condition (except of course for device 1).
+ All colonies that were obtained after transformation were put into culture. One colony was taken into 3,5mL LB medium + Cm at 30µg/mL.
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Revision as of 13:57, 6 July 2016
Thursday 23rd June Lab work Competent cells by Caroline and Lea
A transformation was made with Interlab study constructions using the same protocol as 22/06/2016 but this time with our competent cells. A negative control and pSB1C3 blue plasmids (positive control) were also transformed and displayed.
Interlab study Transformation results by Caroline, Lea and Marion
Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.
Transformed cell cultures by Lea
All colonies that were obtained after transformation were put into culture. One colony was taken into 3,5mL LB medium + Cm at 30µg/mL.
