The 6 clones of each construction had their plasmids extracted using the manual protocol.
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DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted.
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They were resuspended on water with RNase (5µL/mL)
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At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing.