Difference between revisions of "Team:Paris Saclay/Notebook/June/30"

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''By Alice and Lea''
 
''By Alice and Lea''
  
The 6 clones of each construction had their plasmids extracted using the manual protocol.
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DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted.
They were resuspended on water with RNase (5µL/mL)  
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At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing.
 
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{{Team:Paris_Saclay/notebook_footer}}

Revision as of 15:27, 6 July 2016

Thursday 30th June

Lab work

Visualization

Extraction of the plasmids containing gBlocks

By Alice and Lea

DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted. At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing.