Difference between revisions of "Team:Paris Saclay/Notebook/June/30"
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DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted. | DNA from bacteria transformed with plasmids containing gBlocks was extracted following the extraction protocol. For each gblock, DNA of 6 different clones was extracted. | ||
At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing. | At the end, plamids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) to send to sequencing. | ||
| + | |||
| + | ====Digestion of the plasmids containing gBlocks==== | ||
| + | After extraction, plasmids were digested with EcoRI and HindIII | ||
| + | |||
| + | {| class="wikitable" | ||
| + | !- | ||
| + | ! Component | ||
| + | ! Volume (µL) | ||
| + | |- | ||
| + | | Plasmids | ||
| + | | 3 | ||
| + | |- | ||
| + | | Red buffer 10x | ||
| + | | 2 | ||
| + | |- | ||
| + | | Water | ||
| + | | 14 | ||
| + | |- | ||
| + | | EcoRI enzyme | ||
| + | | 0.5 | ||
| + | |- | ||
| + | | HindIII enzyme | ||
| + | | 0.5 | ||
| + | |} | ||
| + | |||
| + | The mix was incubated for 1 hour at 37°C and then stored at -20°C over the night. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 15:51, 6 July 2016
