Difference between revisions of "Team:Paris Saclay/Notebook/June/30"
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====Digestion of the plasmids containing gBlocks==== | ====Digestion of the plasmids containing gBlocks==== | ||
| − | After extraction, plasmids were digested with EcoRI and HindIII | + | ''By Alice and Léa'' |
| + | |||
| + | After extraction, plasmids were digested with EcoRI and HindIII. | ||
{| class="wikitable" | {| class="wikitable" | ||
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The mix was incubated for 1 hour at 37°C and then stored at -20°C over the night. | The mix was incubated for 1 hour at 37°C and then stored at -20°C over the night. | ||
| + | |||
| + | ===Biobrick characterization=== | ||
| + | ====Electrocompetents and transformation by electroporation of BL21==== | ||
| + | ''By Caroline and Charlene'' | ||
| + | |||
| + | The same protocol as 29/06/16 was used for the plasmids K1372001, K1372001+pcl_TAA and the controls which were done following the same protocol but without plasmids. | ||
| + | K1372001 and the controls were streaked on LB + Cm (30µg/mL). K137200 + PCLTAA was streaked on LB + Cm (30µg/mL) + Streptomicin (50µg/mL). | ||
| + | This time two different streaks were made for each plasmid. The first one using 50µL from the culture. The second one was done using 100µL of each remaining culture (400µL) after concentration by centrifugation. | ||
| + | |||
| + | The petri dishes made on 29/06 were stored at 4ºC | ||
| + | |||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 16:04, 6 July 2016
