Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Tuesday 1st July 1.1 Lab Work 1.1.1 Biobrick characterization 1.1.1.1 Buffer preparations Tuesday 1st July Lab Work Biobrick characterization Buffer preparations by Charlene and Caroline Buffer Z (250mL) Buffer Z (250mL) Buffer STOP (15mL) Na2HPO4 – 3,125g NaH2PO4.H2O – 1,38g KCl – 0,185g MgSO4 – 0,04g β-mercaptoethanol – 0,893mL Trisphosphate pH 7,8 1M – 6,25mL MgCl2 2M – 1mL DTT 1M -0,25mL EDTA 0,5M – 0,5mL BSA – 0,25g Glycerol 60% - 62,5mL Triton 10% (100x) – 25mL Na2CO3 1M – 0,78g
by Charlene and Caroline
NaH2PO4.H2O – 1,38g
KCl – 0,185g MgSO4 – 0,04g β-mercaptoethanol – 0,893mL
MgCl2 2M – 1mL DTT 1M -0,25mL EDTA 0,5M – 0,5mL BSA – 0,25g Glycerol 60% - 62,5mL Triton 10% (100x) – 25mL
