Difference between revisions of "Team:Ionis Paris/Protocol 5"
Alexandra m (Talk | contribs) |
|||
| Line 214: | Line 214: | ||
| − | + | <!-- ====START SOCIAL Link==== --> | |
| − | + | <div class="footer_social"> | |
| − | + | <div class="container-fluid"> | |
| − | + | <div class="row"> | |
| − | + | <ul class="ft_top clearfix"> | |
| − | + | <li class="waves-effect waves-light"> | |
| − | + | <a href="https://www.facebook.com/ionisigem/?fref=ts" target="_blank"> | |
| − | + | <i class="zmdi zmdi-facebook "></i>Facebook | |
| − | + | </a> | |
| − | + | </li> | |
| − | + | <li class="waves-effect waves-light"> | |
| − | + | <a href="https://twitter.com/igem_ionis" target="_blank"> | |
| − | + | <i class="zmdi zmdi-twitter"></i>Twitter | |
| − | + | </a> | |
| − | + | </li> | |
| − | + | <li class="waves-effect waves-light"> | |
| − | + | <a href="https://www.youtube.com/channel/UC0RyQsB5YpweSRBYQlAdZDA" target="_blank"> | |
| − | + | <i class="zmdi zmdi-youtube"></i>youtube | |
| − | + | </a> | |
| − | + | </li> | |
| − | + | <li class="waves-effect waves-light"> | |
| − | + | <a href="https://www.linkedin.com/company/igem-ionis" target="_blank"> | |
| − | + | <i class="zmdi zmdi-linkedin"></i>LinkedIn | |
| − | + | </a> | |
| − | + | </li> | |
| − | + | ||
| − | + | </ul> | |
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
| − | + | </div> | |
| − | + | <!-- ====END FOOTER TOP==== --> | |
| − | + | ||
| − | + | <!-- ====START FOOTER AREA==== --> | |
| − | + | <footer class="footer_area"> | |
| − | + | <div class="footer_middle"> | |
| − | + | <div class="container"> | |
| − | + | <div class="row"> | |
| − | + | <div class="col-xs-12"> | |
| − | + | <div class="scroll_area"> | |
| − | + | <div class="sroll_top"> | |
| − | + | <a href="#ancre"> <i class="zmdi zmdi-chevron-up btn waves-effect"> </i> </a> | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | </div> | ||
| + | <div class="row"> | ||
<div class="row"> | <div class="row"> | ||
| − | |||
<div class="col-lg-5 col-md-5 col-sm-12"> | <div class="col-lg-5 col-md-5 col-sm-12"> | ||
<div class="middle_content"> | <div class="middle_content"> | ||
| − | <h4>IONIS | + | <h4>iGEM IONIS</h4> |
| − | <p> We're a group of six different schools from the IONIS Education Group. For this competition we wanted to take advantages of the multiple schools and activity field given by the IONIS education group to create a solid project.</p> | + | <p> We're a group of six different schools from the IONIS Education Group. For this |
| − | + | competition we wanted to take advantages of the multiple schools and activity field | |
| + | given by the IONIS education group to create a solid project.</p> | ||
| + | <a href="https://2016.igem.org/Team:Ionis_Paris/Team">Read More</a> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | + | ||
<div class="col-lg-3 col-lg-offset-1 col-md-4 col-sm-7"> | <div class="col-lg-3 col-lg-offset-1 col-md-4 col-sm-7"> | ||
<div class="footer_Widgets"> | <div class="footer_Widgets"> | ||
| Line 281: | Line 283: | ||
<a href="mailto:igem.ionis@gmail.com">email: igem.ionis@gmail.com</a> | <a href="mailto:igem.ionis@gmail.com">email: igem.ionis@gmail.com</a> | ||
</li> | </li> | ||
| − | + | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 287: | Line 289: | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
| Line 299: | Line 300: | ||
<div class="col-md-7 fix_p_l"> | <div class="col-md-7 fix_p_l"> | ||
<nav> | <nav> | ||
| − | <ul class="ft_bottom"> | + | <ul class="ft_bottom"> |
<li><a href="https://2016.igem.org/Team:Ionis_Paris">Home</a></li> | <li><a href="https://2016.igem.org/Team:Ionis_Paris">Home</a></li> | ||
| − | <li><a href="https://2016.igem.org/ | + | <li><a href="https://2016.igem.org/Team:Ionis_Paris/Working_at_La_Paillasse">in the Lab</a></li> |
| − | <li><a href="https://2016.igem.org/ | + | <li><a href="https://2016.igem.org/Team:Ionis_Paris/Measurement"> Side Projects</a></li> |
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/Team:Ionis_Paris/Parts">Results</a></li> |
| − | <li><a href="https://2016.igem.org/Team:Ionis_Paris/ | + | <li><a href="https://2016.igem.org/Team:Ionis_Paris/HPintro">Human |
| − | <li><a href="https://2016.igem.org/Team | + | Practice</a></li> |
| + | <li><a href="https://2016.igem.org/Team:Ionis_Paris/Team">Team</a></li> | ||
</ul> | </ul> | ||
</nav> | </nav> | ||
| Line 311: | Line 313: | ||
<div class="col-md-5 fix_p"> | <div class="col-md-5 fix_p"> | ||
<div class="ft_paragraph"> | <div class="ft_paragraph"> | ||
| − | <p>©IONIS_IGEM_2016</a>.</p> | + | <p>©IONIS_IGEM_2016</a>.</p> |
</div> | </div> | ||
| − | </div> | + | </div> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | </div> | + | </div> |
| − | + | </footer> | |
| − | + | <!-- ====END FOOTER AREA==== --> | |
| − | + | ||
| − | + | <!-- ====Google Maps API==== --> | |
| − | + | <script src="https://maps.googleapis.com/maps/api/js"></script> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | --> | + | |
| − | + | ||
| + | <!-- Nos scripts --> | ||
| + | <script type="text/javascript" src="https://2016.igem.org/wiki/index.php?title=Template:IONIS_PARIS_JS&action=raw&ctype=text/javascript"></script> | ||
| − | |||
</html> | </html> | ||
Revision as of 11:02, 18 October 2016
Inoculate 100µL of cells in 100mL LB medium
Put 1.11g of CaCl2 in 100mL of sterile H2O to obtain a final concentration of 0.1M of CaCl2 (Molar mass of Cacl2: 110,98g/mol)
Inoculate 3mL of the overnight culture cell from in 90mL of LB. Shake at 37°C until the DO is around 0,6
Protocol 5 : Preparation of competent cells
Aim: Prepare competent bacteria for subsequent transformation
Inoculation
Let the culture grow O/N at 37°C
CaCl2 preparation
Competency
NB : all the following steps must be perform in ice
Transfer cells into sterile ice-cold Falcon tubes
Incubate on ice for 30 min
Spin cells at 4000 rpm for 10 min at 6°C
Discard supernatant and try to drain all remaining media
Gently resuspend in 10mL cold 0,1M CaCl2
Incubate on ice 20 min
Centrifuge 10 min at 4000 rpm at 4°C
Discard supernatant and vortex, gently resuspend on 5mL cold 0,1M CaCl2/15% Glycerol
Transfer in 2mL cryotubes
Store at -80°C for subsequent transformation
