Difference between revisions of "Team:Ionis Paris/Protocol 1"
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Revision as of 11:36, 18 October 2016
Mix the following component in 500mL of water : 5g Tryptone 2.5g Yeast extract 5g NaCl
Add a magnetic stirrer bar and mix the solution until there is no lump left Mix the following component in 500mL of water: 5g Tryptone 2.5g Yeast extract 5g NaCl 7.5g Agar powder
Add a magnetic stirrer bar and mix the solution until no there is lump left
Protocol 1 : Culture Medium preparation
Aim: Make bacteria population by culturing them on specific medium and/or selecting a recombinant strain
Liquid LB medium preparation (500mL)
Autoclave at 121 Celsius degrees for 15 to 30 minutes to sterilize
Allow the autoclaved medium to cool to 55ºC (until you can hold it in your hand) and add antibiotics (Chloramphenicol at a final concentration of 25µg/mL.)
LB Agar plates preparation (500mL)
Autoclave at 121 Celsius degrees for 15 to 30 minutes to sterilize
Allow the autoclaved medium to cool to 55ºC (until you can hold it in your hand) and transfer it into a 50mL Falcon tube*
Add 50µL antibiotics (Chloramphenicol at a final concentration of 25µg/mL) and mix*
Poor 25mL of the liquid into an identified petri plate*
Let the plate dry*
*Steps to realize under the PSM class II
