Difference between revisions of "Team:Ionis Paris/Protocol 2"
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| − | <p><li><a href="http://www.lifescience.net/protocols/15/10x-tae-buffer-10x-tris-acetate-edta">Life science - TAE buffer | + | <p><li><a href="http://www.lifescience.net/protocols/15/10x-tae-buffer-10x-tris-acetate-edta"><font color="DeepPink">Life science - TAE buffer</font></a></li></p> |
| − | + | <p><li><a href="http://chemistry.about.com/od/labrecipes/a/10x-TAE-Electrophoresis-Buffer.htm"><font color="DeepPink">About education - TAE Electrophoresis buffer</font></a></li></p> | |
| − | <p><li><a href="http://chemistry.about.com/od/labrecipes/a/10x-TAE-Electrophoresis-Buffer.htm">About education - TAE Electrophoresis buffer | + | |
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Revision as of 14:59, 18 October 2016
Put 800mL of deionized water in the bottle along with the magnetic bar NB : 1X buffer will contain approximatively 40 mM Tris, 20 mM acetic acid and 1 mM EDTA
Protocol 2 : TAE preparation
Aim: Preparation of the buffer used for electrophoresis
Dissolve 48.5g of Tris base
Add 11.4 mL of glacial acetic acid
Add 20 mL of 0.5M EDTA or 3,7g of EDTA disodium salt
Add deionized water up to 1L
Shake the solution until it becomes homogeneous
Autoclave at 121 Celsius degrees for 15 to 30 minutes to sterilize
Store at room temperature
Be sure to dilute the buffer to 1:10 before us
