Revision as of 14:54, 7 July 2016

Monday 4^th July

By Mathilde and Alice

The following plasmids were digested again with EcoR1 and HindIII using this protocol:

The digestion products were mixed as follow to be migrated on an agarose gel.

Component	Volume (µL)
Digested DNA	20
Loading buffer	3.3

The DNA concentration was still insufficient meaning the extraction step was not efficient.

By Naiane and Laetitia

Plasmids from the following strains were extracted with the kit "Charge Switch-Pro Plasmid Miniprep":

Then plasmids were digested with AvrII

The mix was incubated at 37°C for 1 hour. An electrophoresis was done (0.8% of agarose).

@@ Line 34: / Line 34: @@
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-The mix was incubated at 37°C for 1 hour.
+The digestion products were mixed as follow to be migrated on an agarose gel.
-An electrophoresis was done (0.8% of agarose).
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