Monday 4^th July

Lab work

Visualization

Digestion of plasmids

By Mathilde and Alice

The following plasmids were digested again with EcoR1 and HindIII using this protocol:

• pPS16_001 (from the 6 clones that were selected on 29/06/16)
• pPS16_003 (from the 6 clones that were selected on 29/06/16)
• pPS16_005 (from the 6 clones that were selected on 29/06/16)
• pPS16_006 (from the 6 clones that were selected on 29/06/16)

The digestion products were mixed as follow to be migrated on an agarose gel.

The DNA concentration was still insufficient meaning the extraction step was not efficient.

Bringing DNA closer

Plasmids extraction

By Naiane and Laetitia

Plasmids from the following strains were extracted with the kit "Charge Switch-Pro Plasmid Miniprep":

• DS-NMcas
• DS-SPcasN-
• DS-ST1casN-
• DS-TDCasN-

Then plasmids were digested with AvrII

The mix was incubated at 37°C for 1 hour. An electrophoresis was done (0.8% of agarose).