Difference between revisions of "Team:Austin UTexas/Description"

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<li>Hanke, T., Richhardt, J., Polen, T., Sahm, H., Bringer, S., and Bott, M. (2012) Influence of oxygen limitation, absence of the cytochrome bc1 complex and low pH on global gene expression in Gluconobacter oxydans 621H using DNA microarray technology. <i>Journal of Biotechnology 157</i>, 359–372.</li>
 
<li>Hanke, T., Richhardt, J., Polen, T., Sahm, H., Bringer, S., and Bott, M. (2012) Influence of oxygen limitation, absence of the cytochrome bc1 complex and low pH on global gene expression in Gluconobacter oxydans 621H using DNA microarray technology. <i>Journal of Biotechnology 157</i>, 359–372.</li>
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<li>Kuper, C., and Jung, K. (2005) CadC-mediated activation of the cadBA promoter in Escherichia coli. Journal of Molecular and Microbiological Biotechnology 1, 26–39.</li>
 
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Revision as of 16:33, 18 October 2016

Project Description


Gold Medal Part Characterization

The characterization of the BioBrick P-atp2 from the 2015 BIT-China team was done to see if P-atp2 could be utilized as a basic pH sensor. The results are found here and on the iGEM Registry page, BBa_K1675021


Our Project

Kombucha is a beverage made when a symbiotic community of bacteria and yeast ferments sugared tea. Although kombucha has been consumed for thousands of years in the East, the drink has enjoyed a recent resurgence in popularity [REFERENCE-HOMEPAGE]. Several kombucha breweries operate in Austin, Texas, our team’s hometown. The role microbes play in the production of the beverage has led our team to wonder if synthetic biology could allow us to create “designer kombucha” with enhanced properties, such as more appealing flavors or additional nutrients. In order to do so, our team attempted to isolate the strains responsible for the fermentation of kombucha, identify them, genetically modify them, and add the individual strains into tea media to recreate the drink. We additionally considered potential applications of the ability to genetically modify the microbial population of kombucha, such as reducing the ethanol content of the beverage and improving taste with brazzein, a sweet-tasting protein. AS PART OF OUR HUMAN PRACTICES WE.... [SOMETHING HERE about GMO concerns and alternative direction(s)?]

Click the images below to learn more about our project!


Kombucha Strains

Conjugation

Recapitulation

Ethanol

Brazzein

pH Sensors