Difference between revisions of "Team:SDU-Denmark/Perspectives"
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| + | <div id="overview" style="background-color:#dddddd; padding:0px 10px 10px 20px;"> | ||
| + | <h3 id="top">Overview</h3> | ||
| + | <h5> <img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" width="5%" style="display:inline-block;margin-right:5px;">Bacteriocin results</h5> | ||
| + | <ul class="list"> | ||
| + | <li><i><a href="#purify">Purification of the bacteriocins by the IMPACT method</a></i></li> | ||
| + | <li><i><a href="#determine">Determination of bacteriocin protein concentration using Bradford Protein Assay</a></i></li> | ||
| + | <li><i><a href="#effect">Inhibition of growth of S. aureus MRSA strains and P. aeruginosa detected by MIC</a></i></li> | ||
| + | <li><i><a href="#synergy">Synergistic effect of hybrid bacteriocins detected by MIC</a></i></li></ul> | ||
| + | <h5> <img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" width="5%" style="display:inline-block;margin-right:5px;">Constructing silk fibers</h5> | ||
| + | <ul class="list"> | ||
| + | <li><i><a href="#insert">Successfull insertion of the MaSp1 and MaSp2 gene fragments into pSB1C3</a></i></li> | ||
| + | <li><i><a href="#ligate">Successful ligation test</a></i></li> | ||
| + | <li><i><a href="#prepare">Proof the concept of preparing silk by the ICA method for one monomer of MaSp1 and MaSp2</a></i></li> | ||
| + | <li><i><a href="#dream">Missed ligation of a full construct </a></i></li> | ||
| + | </ul> | ||
| + | <h5> <img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" width="5%" style="display:inline-block;margin-right:5px;">Production optimization of plastic producing cells</h5> | ||
| + | <ul class="list"> | ||
| + | <li><i><a href="#hybrids">Determination of PHB production efficiency of phaCAB library</a></i></li> | ||
| + | <li><i><a href="#optimization">Comparisson of PHB extraction methods</a></i></li> | ||
| + | <li><i><a href="#characterization">iTRAQ analysis of pantothenate kinase II</a></i></li> | ||
| + | <li><i><a href="#secretion">The properties of the secretion system </a></i></li> | ||
| + | |||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | <div id="purify"></div> | ||
| + | <br><h5> <img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="18px" style="display:inline-block;margin-right:5px;margin-bottom:5px;vertical-align:middle;">Bacteriocin purification (<a href="#overview">Top</a>)</h5> | ||
| + | <div> | ||
| + | <div style="float:right;width:60%;padding-left:15px;"><img src="https://static.igem.org/mediawiki/2016/9/9e/T--SDU-Denmark--cPCRThuricinS_bacteriocin.png" alt="MIC test" width="100%"> | ||
| + | <p class="figuretext"><em>Figure 1 shows results of a cPCR originating from respective colonies from transformation of K2018011/pTXB1/E.coli Top10 (ThuricinS), K2018012/pTXB1/E.coli Top10 (LacticinQ) and K2018014/pTXB1/E.coli Top10 (Laterusporulin-ThuricinS). From right; Well 1-3 + 5-7 + 13-14= <a href="http://parts.igem.org/Part:BBa_K2018011" target="_blank">K2018011</a>, Well 4+8 = <a href="http://parts.igem.org/Part:BBa_K2018014" target="_blank">K2018014</a>, Well 9-12 = <a href="http://parts.igem.org/Part:BBa_K2018012" target="_blank">K2018012</a>. The expected length of ThuricinS is 304 bp. <a href="https://static.igem.org/mediawiki/2016/e/ea/T--SDU-Denmark--ThermoFisher_Generuler_DNA_ladder.jpeg" target="_blank">GeneRulerTM 100 bp DNA ladder </a> were used <span class="tooltip"><span class="tooltiptext"><a href="https://www.thermofisher.com/dk/en/home/brands/thermo-scientific/molecular-biology/thermo-scientific-nucleic-acid-electrophoresis-purification/dna-electrophoresis-thermo-scientific/dna-ladders-thermo-scientific/generuler-dna-ladders.html#" target="_blank">ThermoFisher scientific. </a></span></span>.</p></em></div> | ||
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<!--- END CONTENT------------------------------------> | <!--- END CONTENT------------------------------------> | ||
Revision as of 20:11, 18 October 2016
Perspectives
Overview
Bacteriocin results
- Purification of the bacteriocins by the IMPACT method
- Determination of bacteriocin protein concentration using Bradford Protein Assay
- Inhibition of growth of S. aureus MRSA strains and P. aeruginosa detected by MIC
- Synergistic effect of hybrid bacteriocins detected by MIC
Constructing silk fibers
- Successfull insertion of the MaSp1 and MaSp2 gene fragments into pSB1C3
- Successful ligation test
- Proof the concept of preparing silk by the ICA method for one monomer of MaSp1 and MaSp2
- Missed ligation of a full construct
Production optimization of plastic producing cells
Bacteriocin purification (Top)
Figure 1 shows results of a cPCR originating from respective colonies from transformation of K2018011/pTXB1/E.coli Top10 (ThuricinS), K2018012/pTXB1/E.coli Top10 (LacticinQ) and K2018014/pTXB1/E.coli Top10 (Laterusporulin-ThuricinS). From right; Well 1-3 + 5-7 + 13-14= K2018011, Well 4+8 = K2018014, Well 9-12 = K2018012. The expected length of ThuricinS is 304 bp. GeneRulerTM 100 bp DNA ladder were used ThermoFisher scientific. .
