Difference between revisions of "Team:Freiburg/Lab"

Revision as of 20:18, 18 October 2016

Lab Summary

Without a lab ….there is no iGEM. Luckily we had the opportunity to work in the lab of our main sponsor, the BIOSS. They didn't just provide the lab but also a lot of their professional equipment like the FACS, the fluorescence microscope and personal support. They kindly teached us the handling of the equipment. Without the BIOSS our great project would not have been possible. Fortunately they also provided us access to the kitchen of the institute. Otherwise possibly the half of our team members would have been starving :-).

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+{{Freiburg/Away}}
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          <h5 style="text-align: center"> Lab Summary </h5>
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-            <center><img  style="width:350px" src="https://static.igem.org/mediawiki/2016/c/c3/T--Freiburg--Lab0004.png"> </center><font color="#74e4f7">
-This year our team was working with the model organisms <i>Bacillus subtilis</i> and <i>Escherichia coli</i>. Since <i>E. coli</i> is commonly used in many laboratories, we had no problem handling it. Apparently, there are only a few people who are working with <i>Bacillus subtilis</i>, but we kindly received some help and protocols from former iGEM team Munich 2012 and support from the Bacillus Genetic Stock Center (BGSC) from Ohio who always answered our numerous questions. <font color="#74e4f7">
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+<center><img  style="width:350px" src="https://static.igem.org/mediawiki/2016/c/c3/T--Freiburg--Lab0004.png"> </center>
+<font color="#74e4f7">
+Without a lab ….there is no iGEM. Luckily we  had  the opportunity to work in the lab of our main sponsor, the BIOSS. They didn't just provide the lab but also a lot of their professional equipment like the FACS, the fluorescence microscope and personal support. They kindly teached us the handling of the equipment.
+Without the BIOSS our great project would not have been possible.
+Fortunately they also provided us access to the kitchen of the institute. Otherwise possibly the half of our team members would have been starving :-).
-We want to use the endospores of <i>Bacillus subtilis</i> as a carrier vessel for a prodrug activating enzyme and a nanobody to improve the targeted drug delivery system.
+<center><img style="width:350px" src="https://static.igem.org/mediawiki/2016/9/96/T--Freiburg--LaboreSued.jpg" > </center>
-        <center><img style="width:350px" src="https://static.igem.org/mediawiki/2016/9/96/T--Freiburg--LaboreSued.jpg" > </center>
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-<i>Bacillus subtilis</i> builds these spores under distressed conditions (such as nutrition deficiency). The spores are highly resistant to heat, UV-light and are easy to handle.
-<br><br>
-We modified the spores in their coat proteins so that they are expressing the fusion proteins, the Glutathione S-Transferase (GST) and nanobody, on their surface. We experimented with modifications on the genes CotZ, CgeA,CotG and CotB, to see where we could achieve the optimal conditions.
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