Difference between revisions of "Team:Alverno CA/Proof"
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| − | <p>< | + | <p><h1>Proof of Concept</h1></p> |
<p>We constructed a set of RFP/GFP reporters (BBa_K2145000-BBa_K2145003) with a large spacer between the two reporter genes. The reporter set includes all of the combinations of orientations of the two genes, and will demonstrate orientation-dependent expression interference between genes on the same plasmid. This set of constructs demonstrates the need for new techniques for insulating the components of multi-gene circuits on the same piece of DNA.</p> | <p>We constructed a set of RFP/GFP reporters (BBa_K2145000-BBa_K2145003) with a large spacer between the two reporter genes. The reporter set includes all of the combinations of orientations of the two genes, and will demonstrate orientation-dependent expression interference between genes on the same plasmid. This set of constructs demonstrates the need for new techniques for insulating the components of multi-gene circuits on the same piece of DNA.</p> | ||
Revision as of 21:09, 18 October 2016
Proof of Concept
We constructed a set of RFP/GFP reporters (BBa_K2145000-BBa_K2145003) with a large spacer between the two reporter genes. The reporter set includes all of the combinations of orientations of the two genes, and will demonstrate orientation-dependent expression interference between genes on the same plasmid. This set of constructs demonstrates the need for new techniques for insulating the components of multi-gene circuits on the same piece of DNA.
