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<h5>8. Record the data, specifically volume of preloading culture and preloading media from the table in the notebook.</h5> | <h5>8. Record the data, specifically volume of preloading culture and preloading media from the table in the notebook.</h5> | ||
<h5>9. Dilute accordingly (media is LB media with antibiotic) in a new 96 well plate (if needed). Usually about 500uL per well.</h5> | <h5>9. Dilute accordingly (media is LB media with antibiotic) in a new 96 well plate (if needed). Usually about 500uL per well.</h5> | ||
− | <h5>10. Place plate back into | + | <h5>10. Place plate back into reader and set to OD-RFP-GFP protocol (made in plate reader) and start run.</h5> |
− | <h5>11. Let it run overnight (120 runs total with about | + | <h5>11. Let it run overnight (120 runs total with about 3 minute intervals) and check in the morning. </h5> |
<h5>12. Import data results into Excel spreadsheet.</h5> | <h5>12. Import data results into Excel spreadsheet.</h5> | ||
− | <h5>13. Upload to Google drive.</h5> | + | <h5>13. Optional: Upload to Google drive. </h5> |
<h5>14. To analyze data using Python program—file must be in a csv format. (If you would like the code for analyzing this type of data, please contact us!)</h5> | <h5>14. To analyze data using Python program—file must be in a csv format. (If you would like the code for analyzing this type of data, please contact us!)</h5> | ||
<h3>Purification of Plasmid DNA</h3> | <h3>Purification of Plasmid DNA</h3> | ||
− | <h4>Ingredients/Materials * </h4> | + | <h4>Ingredients/Materials *</h4> |
<h5>• Liquid cultures</h5> | <h5>• Liquid cultures</h5> | ||
<h5>• Miniprep DNA kit (we used BioBasic kit)</h5> | <h5>• Miniprep DNA kit (we used BioBasic kit)</h5> |
Revision as of 02:52, 19 October 2016