Difference between revisions of "Team:Newcastle/Notebook/Lab/Lightbulb/Week 14"
| Line 62: | Line 62: | ||
<li>BL21 negative control</li> | <li>BL21 negative control</li> | ||
</ul> | </ul> | ||
| + | <h2>September 20th 2016 </h2> | ||
| + | <p>We started out by setting up a plate using the same layout as previously described in order to test our ‘light bulb’ constructs, using the fresh cultures we inoculated the day before. We used a 96 well plate with clear bottoms and black sides in order to measure both OD600 and fluorescence. We diluted the concentrated cell mixture to an appropriate OD600 value of around 0.05, which will allow us to see a full growth curve. We used a plate reader with the gain set to 528 (as used in the inter-lab study), measuring fluorescence at two wavelengths, an excitation of 485nm for both and emissions of 518nm and 538nm respectively. Over the next few days we plan to test the constructs in the plate reader at 30oC, 37oC and 42oC over a 24-hour period. Today we tested at 37oC. Inoculated fresh LB culture for use on the following day. | ||
| + | |||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 08:17, 19 October 2016
September 19th 2016
We inoculated fresh liquid LB broth for use with the plate reader and testing the battery constructs:
- Battery 1
- Battery 2
- DNA2.0 light construct
- Alternative light construct
- Variable Resistor (tagged)
- Variable Resistor (un-tagged)
- Positive Control (from InterLab study)
- Sample 1 (from InterLab study)
- Negative Control (from InterLab study)
- BL21 negative control
September 20th 2016
We started out by setting up a plate using the same layout as previously described in order to test our ‘light bulb’ constructs, using the fresh cultures we inoculated the day before. We used a 96 well plate with clear bottoms and black sides in order to measure both OD600 and fluorescence. We diluted the concentrated cell mixture to an appropriate OD600 value of around 0.05, which will allow us to see a full growth curve. We used a plate reader with the gain set to 528 (as used in the inter-lab study), measuring fluorescence at two wavelengths, an excitation of 485nm for both and emissions of 518nm and 538nm respectively. Over the next few days we plan to test the constructs in the plate reader at 30oC, 37oC and 42oC over a 24-hour period. Today we tested at 37oC. Inoculated fresh LB culture for use on the following day.
