Difference between revisions of "Team:Paris Saclay/Notebook/July/11"
(→Biobrick characterization) |
(→Electrocompetents and transformation by electroporation of BL21) |
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''By Charlène'' | ''By Charlène'' | ||
| − | BL21 cells were grown up to OD<sub>600nm</sub>=7.14. They were then [[Team:Paris_Saclay/Experiments#ElectroCompetent|made competent and transformed]] with K1372001 extracted from clone 2 and pcl_TAA, pcl_TAG or pcl_Tq to | + | BL21 cells were grown up to OD<sub>600nm</sub>=7.14. They were then [[Team:Paris_Saclay/Experiments#ElectroCompetent|made competent and transformed]] with K1372001 extracted from clone 2 and pcl_TAA, pcl_TAG or pcl_Tq to create the following strains: |
*BL21|K1372001+pcl_TAA | *BL21|K1372001+pcl_TAA | ||
*BL21|K1372001+pcl_TAG | *BL21|K1372001+pcl_TAG | ||
*BL21|K1372001+pcl_Tq | *BL21|K1372001+pcl_Tq | ||
Revision as of 16:13, 11 July 2016
