Thursday 23^rd June

Lab work

Heat-shock competent cells

By Caroline and Lea

The cells competency was checked by transforming cells as usual with two control plasmids:

• the Interlab study negative control
• pSB1C3 blue

Interlab study

Transformation results

By Caroline, Lea and Marion

Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the transformed cells obtained on 22/06/2016 were spread entirely.

Transformed cell cultures

By Lea

All colonies that were obtained after transformation were put into culture. One colony was taken into 3.5mL LB medium + Cm at 30µg/mL.