Wednesday 13^th July

Lab work

Getting DNA closer

Glycerol stocks for DH5α tra,sformed with DS-NMcas, DS-SPcasN-, DS-ST1casN- and DS-TDcasN-

By Laetitia

8 stocks were made: 2 clones (Cl 1 and Cl 2) by cas:

• Cl1 and Cl2 of DS-NMcas
• Cl1 and Cl2 of DS-SPcasN-
• Cl1 and Cl2 of DS-ST1casN-
• Cl1 and Cl2 of DS-TDcasN-

For 1 glycerol stock: 1mL of liquid culture 500 μL of glycerol 60%

Biobrick characterization

BL21 electrocompetent cells preparation and transformation

By Mathilde and Charlène

We did an electro-transformation of BL21 with :

• pcl_TAA + K1372001 (time constant equal to 5.9ms)
• pcl_TAG + K1372001 (time constant equal to 6 ms)
• pcl_Tq + K1372001 (time constant equal to 6 ms)

Cells were displayed on LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. For each condition, we made a dish with 50µL of cells and another with 500µL of cells.

Visualization