Difference between revisions of "Team:BIT/Human Practices"

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awesome.min.css">
 
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#home{
 
#home{
 
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background: url(https://static.igem.org/mediawiki/2016/9/97/BIT-BIology.jpg);
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<li><a href="https://2016.igem.org/Team:BIT">Home</a></li>
 
<li><a href="https://2016.igem.org/Team:BIT">Home</a></li>
 
<li><a href="https://2016.igem.org/Team:BIT/Biology">Project</a></li>
 
<li><a href="https://2016.igem.org/Team:BIT/Biology">Project</a></li>
−
<li><a href="https://2016.igem.org/Team:BIT/Human_Practices" >Practice</a></li>
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<li><a href="https://2016.igem.org/Team:BIT/Human_Practices" ><span class="style1">Practice</span></a></li>
−
<li><a href="https://2016.igem.org/Team:BIT/Judging_Form" ><span class="style1">Judging</span></a></li>
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<li><a href="https://2016.igem.org/Team:BIT/Judging_Form" >Judging</a></li>
 
                 <li><a href="https://2016.igem.org/Team:BIT/Parts_Submission">Parts</a></li>
 
                 <li><a href="https://2016.igem.org/Team:BIT/Parts_Submission">Parts</a></li>
 
                 <li><a href="https://2016.igem.org/Team:BIT/HP/Silver">Attrievement</a></li>
 
                 <li><a href="https://2016.igem.org/Team:BIT/HP/Silver">Attrievement</a></li>
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<div class="content">
 
<div class="content">
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<h4>JUDGING</h4>
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<h4>PRACTICE</h4>
 
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
 
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
 
<div class="header-text btn">
 
<div class="header-text btn">
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<h1><font color=#c91f77>I</font>NTERLAB <font color=#c91f77>S</font>TUDY</h1>
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<h1><font color=#c91f77>H</font>UMAN <font color=#c91f77>P</font>RACTICES</h1>
 
</div>
 
</div>
 
</div>
 
</div>
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<div id="subNav">
 
<div id="subNav">
 
   <ul>
 
   <ul>
−
     <li><a href="https://2016.igem.org/Team:BIT/Judging_Form"><h2>Judging Form</h2></a></li>
+
     <li class=color1><a href="https://2016.igem.org/Team:BIT/Human_Practices"><h2>Human Practices</h2></a></li>
−
     <li><a href="https://2016.igem.org/Team:BIT/Safety_Form"><h2>Safety Form</h2></a></li>
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     <li><a href="https://2016.igem.org/Team:BIT/Collaborations"><h2>Collaborations</h2></a></li>
−
     <li class=color1><a href="https://2016.igem.org/Team:BIT/Interlab_Study"><h2>Interlab Study</h2></a></li>
+
     <li><a href="https://2016.igem.org/Team:BIT/Meet_ups"><h2>Meet ups</h2></a></li>
 
   </ul>
 
   </ul>
 
</div>
 
</div>
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                 <div id="words" style="float:right;width:70%">
 
                 <div id="words" style="float:right;width:70%">
 
                 <div class="row text-center">
 
                 <div class="row text-center">
−
<h2><br><br><br><br><br><br><br>Questionnaire</h2>
+
<h2><br><br><br><br><br><br><br>Summary</h2>
 
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
 
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
 
</div>
 
</div>
 
                 <div class="indent">
 
                 <div class="indent">
 
<p>
 
<p>
−
This year we issued the questionnaire through the Internet, with both Chinese and English versions and got information after collecting them. The questions were centered on the personal health, physical examination, the understanding of breast cancer and the team’s products. It was distributed online in January and ended in March and we received 299 valid feedbacks. <br><br></p>
+
The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). Our Interlab work was carried out with the DH5-alpha as an expression host, and the Thermo Scientific Varioskan Flash spectral scanning as the equipment. We transfer the five reporter constructs into E.coli cells separately. And we measure the fluorescence data and optical density data with spectral scanning machine every 1 hour during a 6 hours cultivation. <br><br></p>
  
−
             
+
                <div class="row text-center">
−
+
<h2><br><br><br><br><br><br><br>Methods</h2>
−
<p><b>
+
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
−
Survey<br><br>
+
</div>
−
</b></p>
+
<div class="indent">
 
<p>
 
<p>
−
PARTⅠ Identity information<br><br>
+
We use endonuclease (EcoRI and PstI, XbaI and PstI) and T4 DNA ligase (NEB) to construct them. Finally, the ligation product was introduced into the pSB1C3. All constructs used were transformed into DH5α cells.<br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
Q1. What is your gender?
+
The cultivation of our bacteria was performed in tubes filled with 7 mL LB liquid medium. The cultures were kept at 37 °C and 110 rpm in shaker. <br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
A. Male
+
Antibiotics were added to each media (chloramphenicol 25mg/mL for Positive control、Negative control、Device 1：J23101+I13504、Device 2:J23106+I13504、Device 3:J23117+I13504). <br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
B. Female
+
We used100mL Luria Broth Liquid media(102μg/mL chloramphenicol) in 250 mL conical flask to grow our cells(37℃，110rmp). Every 1h, we took 100μL in 96-well plate (round wells, flat bottom) to test the OD600 and fluorescence intensity. <br><br>
 
</p>
 
</p>
−
<img src="https://static.igem.org/mediawiki/2016/b/bd/BIT-PAPER1.gif" class="img-responsive" alt="..."><br><br>
 
 
<p>
 
<p>
−
Q2. How old are you?
+
Measurement of fluorescence and OD were performed using the Thermo Scientific Varioskan Flash with the 'Thermo Scientific SkanIt' software and with the 96-Well Multiwell Plates (Corning). <br><br>
 
</p>
 
</p>
−
<p>
+
<div class="container">
−
A. Below20
+
                <div id="words" style="float:right;width:70%">
−
</p>
+
                <div class="row text-center">
−
<p>
+
<h2><br><br><br><br><br><br><br>Photometry</h2>
−
B. 21-30
+
<center><hr width="70%" align="center" color="#c91f77" size=1;></hr></center>
−
</p>
+
</div>  
−
<p>
+
−
C. 31-40
+
−
</p>
+
−
<p>  
+
−
D. Above41
+
−
</p>
+
−
<img src="https://static.igem.org/mediawiki/2016/b/b0/BIT-PAPER2.gif" class="img-responsive" alt="..."><br><br>
+
  
 +
<img src="https://static.igem.org/mediawiki/2016/b/bb/BIT_Table_Photometry.png" class="img-responsive" alt="...">
 
<p>
 
<p>
−
Q3. What is your record of formal schooling?*
+
Delegates from seven Portuguese speaking countries — Portugal, Brazil, Cape Verde, Guinea-Bissau, Mozambique, Angola and East Timor — attended the forum.<br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
A. Junior high school
+
Before the ceremony, Li launched a trade and economic cooperation program to promote further exchanges between China and Portuguese-speaking countries.
 +
<br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
B. High school
+
China and the Portuguese-speaking countries contribute 17 percent of the world economy and 22 percent of global population, with varying advantages in technology, capital and market practices, Li said.<br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
C. College
+
The participating countries should consolidate political trust, promote trade liberalization and investment and strengthen cooperation in production capacity and people-to-people exchanges, the premier told the forum.<br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
D. Undergraduate or over it
+
China's economic restructuring and upgrading will bring great investment opportunities and market potential, creating bright prospects for businesses from Portuguese-speaking countries, Li said.<br><br>
 
</p>
 
</p>
−
<img src="https://static.igem.org/mediawiki/2016/4/41/BIT-PAPER3.gif" class="img-responsive" alt="..."><br><br>
 
 
<p>
 
<p>
−
Q4. Have you been married?*
+
He said the trade volume between China and Portuguese-speaking countries reached $100 billion last year — about 10 times of that of 2003.<br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
A. YES
+
Cape Verde's Prime Minister Ulisses Correia e Silva said the Macao forum injected new momentum into strategic cooperation between all the participating countries. He added that his country will align with China's Belt and Road Initiative and make use of the Macao forum to bring more commodities to Africa.<br><br>
 
</p>
 
</p>
 
<p>
 
<p>
−
B. NO
+
The prime ministers of Guinea-Bissau and Mozambique vowed to adopt favorable policies and improve the rule of law to attract investors.<br><br>
 
</p>
 
</p>
−
<img src="https://static.igem.org/mediawiki/2016/1/14/BIT-PAPER4.gif" class="img-responsive" alt="..."><br><br>
 
−
<p>
 
−
Q5. What is your salary each month?*
 
−
A. ￥0
 
−
</p>
 
−
<p>
 
−
B. ￥0-3000
 
−
</p>
 
−
<p>
 
−
C. ￥3000-10000
 
−
</p>
 
−
<p>
 
−
D. Above￥10000
 
−
</p>
 
−
<img src="https://static.igem.org/mediawiki/2016/6/64/BIT-PAPER5.gif" class="img-responsive" alt="..."><br><br>
 
−
<p>
 
−
Q6. Is the field you are engaged in is connected to the biological?*
 
−
</p>
 
−
<p>
 
−
A. YES
 
−
</p>
 
−
<p>
 
−
B. NO
 
−
</p>
 
−
<img src="https://static.igem.org/mediawiki/2016/e/eb/BIT-PAPER6.gif" class="img-responsive" alt="..."><br><br>
 
−
<p>
 
−
Q7. How much do you cost each month?*
 
−
</p>
 
−
<p>
 
−
A. Below￥1000
 
−
</p>
 
−
<p>
 
−
B. ￥1000-2000
 
−
</p>
 
−
<p>
 
−
C. ￥2000-3000
 
−
</p>
 
−
<p>
 
−
D. Above￥3000
 
−
</p>
 
−
<img src="https://static.igem.org/mediawiki/2016/a/a3/BIT-PAPER7.gif" class="img-responsive" alt="..."><br><br>
 
−
<p>
 
−
Q8. Have you bought social security or medical insurance?*
 
−
</p>
 
−
<p>
 
−
A. YES
 
−
</p>
 
−
<p>
 
−
B. NO
 
−
</p>
 
−
<br>
 
−
<img src="https://static.igem.org/mediawiki/2016/1/16/BIT-PAPER8.gif" class="img-responsive" alt="..."><br><br>
 
−
 
−
PART Ⅱ Physical condition
 
−
 
−
<p>
 
−
Q9. What is your figure?*
 
−
</p>
 
−
<p>
 
−
A. Thin
 
−
</p>
 
−
<p>
 
−
B. Normal
 
−
</p>
 
−
<p>
 
−
C. Fat
 
−
</p>
 
−
<img src="https://static.igem.org/mediawiki/2016/6/64/BIT-PAPER9.gif" class="img-responsive" alt="..."><br><br>
 
−
 
 
<p>
 
<p>
 
Deng Yanzi contributed to this story<br><br><br><br><br><br>
 
Deng Yanzi contributed to this story<br><br><br><br><br><br>

Revision as of 16:36, 19 October 2016

<!DOCTYPE html> Biology

PRACTICE

HUMAN PRACTICES








Summary

The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). Our Interlab work was carried out with the DH5-alpha as an expression host, and the Thermo Scientific Varioskan Flash spectral scanning as the equipment. We transfer the five reporter constructs into E.coli cells separately. And we measure the fluorescence data and optical density data with spectral scanning machine every 1 hour during a 6 hours cultivation.








Methods

We use endonuclease (EcoRI and PstI, XbaI and PstI) and T4 DNA ligase (NEB) to construct them. Finally, the ligation product was introduced into the pSB1C3. All constructs used were transformed into DH5α cells.

The cultivation of our bacteria was performed in tubes filled with 7 mL LB liquid medium. The cultures were kept at 37 °C and 110 rpm in shaker.

Antibiotics were added to each media (chloramphenicol 25mg/mL for Positive control、Negative control、Device 1：J23101+I13504、Device 2:J23106+I13504、Device 3:J23117+I13504).

We used100mL Luria Broth Liquid media(102μg/mL chloramphenicol) in 250 mL conical flask to grow our cells(37℃，110rmp). Every 1h, we took 100μL in 96-well plate (round wells, flat bottom) to test the OD600 and fluorescence intensity.

Measurement of fluorescence and OD were performed using the Thermo Scientific Varioskan Flash with the 'Thermo Scientific SkanIt' software and with the 96-Well Multiwell Plates (Corning).








Photometry

...

Delegates from seven Portuguese speaking countries — Portugal, Brazil, Cape Verde, Guinea-Bissau, Mozambique, Angola and East Timor — attended the forum.

Before the ceremony, Li launched a trade and economic cooperation program to promote further exchanges between China and Portuguese-speaking countries.

China and the Portuguese-speaking countries contribute 17 percent of the world economy and 22 percent of global population, with varying advantages in technology, capital and market practices, Li said.

The participating countries should consolidate political trust, promote trade liberalization and investment and strengthen cooperation in production capacity and people-to-people exchanges, the premier told the forum.

China's economic restructuring and upgrading will bring great investment opportunities and market potential, creating bright prospects for businesses from Portuguese-speaking countries, Li said.

He said the trade volume between China and Portuguese-speaking countries reached $100 billion last year — about 10 times of that of 2003.

Cape Verde's Prime Minister Ulisses Correia e Silva said the Macao forum injected new momentum into strategic cooperation between all the participating countries. He added that his country will align with China's Belt and Road Initiative and make use of the Macao forum to bring more commodities to Africa.

The prime ministers of Guinea-Bissau and Mozambique vowed to adopt favorable policies and improve the rule of law to attract investors.

Deng Yanzi contributed to this story





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