Difference between revisions of "Team:BIT/Human Practices"

Line 174: Line 174:
 
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                 <div id="words" style="float:right;width:70%">
 
                 <div class="row text-center">
 
                 <div class="row text-center">
−
<h2><br><br><br><br><br><br><br>Summary</h2>
+
<h2><br><br><br><br><br><br><br>Questionnaire</h2>
 
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
 
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
 
</div>
 
</div>
 
                 <div class="indent">
 
                 <div class="indent">
 
<p>
 
<p>
−
The objective of this year’s iGEM Interlab study is to quantify expression of five different reporter constructs. These constructs have GFP under the control of different promoter and ribosome binding sequences (RBS). Our Interlab work was carried out with the DH5-alpha as an expression host, and the Thermo Scientific Varioskan Flash spectral scanning as the equipment. We transfer the five reporter constructs into E.coli cells separately. And we measure the fluorescence data and optical density data with spectral scanning machine every 1 hour during a 6 hours cultivation. <br><br></p>
+
This year we issued the questionnaire through the Internet, with both Chinese and English versions and got information after collecting them. The questions were centered on the personal health, physical examination, the understanding of breast cancer and the team’s products. It was distributed online in January and ended in March and we received 299 valid feedbacks. <br><br></p>
  
−
                <div class="row text-center">
+
             
−
<h2><br><br><br><br><br><br><br>Methods</h2>
+
<div class="row text-center">
 +
<h2><br><br><br><br><br><br><br>Survey</h2>
 
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
 
<center><hr width="70%"  align="center"  color="#c91f77" size=1;></hr></center>
−
</div>  
+
</div>
−
<div class="indent">
+
 
 +
<p><b>
 +
PARTⅠ Identity information<br><br>
 +
</b></p>
 
<p>
 
<p>
−
We use endonuclease (EcoRI and PstI, XbaI and PstI) and T4 DNA ligase (NEB) to construct them. Finally, the ligation product was introduced into the pSB1C3. All constructs used were transformed into DH5α cells.<br><br>
+
Q1. What is your gender?
 
</p>
 
</p>
 +
A. Male
 +
<br>
 +
B. Female
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/b/bd/BIT-PAPER1.gif" class="img-responsive" alt="..."><br><br>
 
<p>
 
<p>
−
The cultivation of our bacteria was performed in tubes filled with 7 mL LB liquid medium. The cultures were kept at 37 °C and 110 rpm in shaker. <br><br>
+
Q2. How old are you?
 
</p>
 
</p>
 +
A. Below20
 +
<br>
 +
B. 21-30
 +
<br>
 +
C. 31-40
 +
<br>
 +
D. Above41
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/b/b0/BIT-PAPER2.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
Antibiotics were added to each media (chloramphenicol 25mg/mL for Positive control、Negative control、Device 1：J23101+I13504、Device 2:J23106+I13504、Device 3:J23117+I13504). <br><br>
+
Q3. What is your record of formal schooling?*
 
</p>
 
</p>
 +
A. Junior high school
 +
<br>
 +
B. High school
 +
<br>
 +
C. College
 +
<br>
 +
D. Undergraduate or over it
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/4/41/BIT-PAPER3.gif" class="img-responsive" alt="..."><br><br>
 
<p>
 
<p>
−
We used100mL Luria Broth Liquid media(102μg/mL chloramphenicol) in 250 mL conical flask to grow our cells(37℃，110rmp). Every 1h, we took 100μL in 96-well plate (round wells, flat bottom) to test the OD600 and fluorescence intensity. <br><br>
+
Q4. Have you been married?*
 
</p>
 
</p>
 +
<br>
 +
A. YES
 +
<br>
 +
B. NO
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/1/14/BIT-PAPER4.gif" class="img-responsive" alt="..."><br><br>
 
<p>
 
<p>
−
Measurement of fluorescence and OD were performed using the Thermo Scientific Varioskan Flash with the 'Thermo Scientific SkanIt' software and with the 96-Well Multiwell Plates (Corning). <br><br>
+
Q5. What is your salary each month?*
 
</p>
 
</p>
−
<div class="container">
+
A. ￥0
−
                <div id="words" style="float:right;width:70%">
+
<br>
−
                <div class="row text-center">
+
B. ￥0-3000
−
<h2><br><br><br><br><br><br><br>Photometry</h2>
+
<br>
−
<center><hr width="70%" align="center" color="#c91f77" size=1;></hr></center>
+
C. ￥3000-10000
−
</div>  
+
<br>
 +
D. Above￥10000
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/6/64/BIT-PAPER5.gif" class="img-responsive" alt="..."><br><br>
 +
<p>
 +
Q6. Is the field you are engaged in is connected to the biological?*
 +
</p>
 +
A. YES
 +
<br>
 +
B. NO
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/e/eb/BIT-PAPER6.gif" class="img-responsive" alt="..."><br><br>
 +
<p>
 +
Q7. How much do you cost each month?*
 +
</p>
 +
A. Below￥1000
 +
<br>
 +
B. ￥1000-2000
 +
<br>
 +
C. ￥2000-3000
 +
<br>
 +
D. Above￥3000
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/a/a3/BIT-PAPER7.gif" class="img-responsive" alt="..."><br><br>
 +
<p>
 +
Q8. Have you bought social security or medical insurance?*
 +
</p>
 +
A. YES
 +
<br>
 +
B. NO
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/1/16/BIT-PAPER8.gif" class="img-responsive" alt="..."><br><br>
  
−
<img src="https://static.igem.org/mediawiki/2016/b/bb/BIT_Table_Photometry.png" class="img-responsive" alt="...">
+
<p><b>
 +
PART Ⅱ Physical condition
 +
</b></p>
 
<p>
 
<p>
−
Delegates from seven Portuguese speaking countries — Portugal, Brazil, Cape Verde, Guinea-Bissau, Mozambique, Angola and East Timor — attended the forum.<br><br>
+
Q9. What is your figure?*
 
</p>
 
</p>
 +
A. Thin
 +
<br>
 +
B. Normal
 +
<br>
 +
C. Fat
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/6/64/BIT-PAPER9.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
Before the ceremony, Li launched a trade and economic cooperation program to promote further exchanges between China and Portuguese-speaking countries.
+
Q10. What is your physical quality?*
 +
</p>
 +
A. Bad
 +
<br>
 +
B. Normal
 +
<br>
 +
C. Well
 
<br><br>
 
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/7/76/BIT-PAPER10.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q11. How often do you do exercise each week?*
 
</p>
 
</p>
 +
A. Never
 +
<br>
 +
B. 1-2times
 +
<br>
 +
C. Above 3 times
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/7/7b/BIT-PAPER11.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
China and the Portuguese-speaking countries contribute 17 percent of the world economy and 22 percent of global population, with varying advantages in technology, capital and market practices, Li said.<br><br>
+
Q12. Why do you exercise?*
 
</p>
 
</p>
 +
A. For hobby
 +
<br>
 +
B. Someone organize
 +
<br>
 +
C. Keep fit
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/e/ef/BIT-PAPER12.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
The participating countries should consolidate political trust, promote trade liberalization and investment and strengthen cooperation in production capacity and people-to-people exchanges, the premier told the forum.<br><br>
+
Q13. How long do you exercise each time?*
 
</p>
 
</p>
 +
A. 10-20minutes
 +
<br>
 +
B. 20-30minutes
 +
<br>
 +
C. Above30minutes
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/f/ff/BIT-PAPER13.gif.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
China's economic restructuring and upgrading will bring great investment opportunities and market potential, creating bright prospects for businesses from Portuguese-speaking countries, Li said.<br><br>
+
Q14.Have you ever had significant medical history?*
 
</p>
 
</p>
 +
A. YES
 +
<br>
 +
B. NO
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/c/cb/BIT-PAPER14.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
He said the trade volume between China and Portuguese-speaking countries reached $100 billion last year — about 10 times of that of 2003.<br><br>
+
Q15.When do you go to bed?*
 
</p>
 
</p>
 +
A. Before10:00P.M
 +
<br>
 +
B. 10:00P.M-12:00P.M
 +
<br>
 +
C. After12:00P.M
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/7/75/BIT-PAPER15.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
Cape Verde's Prime Minister Ulisses Correia e Silva said the Macao forum injected new momentum into strategic cooperation between all the participating countries. He added that his country will align with China's Belt and Road Initiative and make use of the Macao forum to bring more commodities to Africa.<br><br>
+
Q16. Have you ever smoked or drunk?*
 
</p>
 
</p>
 +
A. Neither
 +
<br>
 +
B. Smoked
 +
<br>
 +
C. Drunk
 +
<br>
 +
D. Both
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/d/de/BIT-PAPER16.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
The prime ministers of Guinea-Bissau and Mozambique vowed to adopt favorable policies and improve the rule of law to attract investors.<br><br>
+
Q17. Do you have breakfast?*
 
</p>
 
</p>
 +
A. Never
 +
<br>
 +
B. Sometimes
 +
<br>
 +
C. Always
 +
<br>
 +
D. Everyday
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/6/63/BIT-PAPER17.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p><b>
 +
PART Ⅲ About medical
 +
</b></p>
 +
<p>
 +
Q18.How often do you do a physical examination ?*
 +
</p>
 +
A. Every year
 +
<br>
 +
B. Not regular
 +
<br>
 +
C. Never
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/c/c8/BIT-PAPER18.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q19. What do you think about the price of medical?*
 +
</p>
 +
A. Expensive
 +
<br>
 +
B. Normal
 +
<br>
 +
C. Cheap
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/3/38/BIT-PAPER19.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q20. What is the meaning of medical?*
 +
</p>
 +
A. Predict
 +
<br>
 +
B. Check disease
 +
<br>
 +
C. Other
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/b/bb/BIT-PAPER20.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q21. Can you understand the medical report?*
 +
</p>
 +
A. YES
 +
<br>
 +
B. NO
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2016/e/e6/BIT-PAPER21.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p><b>
 +
PART Ⅳ About breast cancer
 +
</b></p>
 +
<p>
 +
Q22.Which way do you use to know about breast cancer?*(multiple choice)
 +
</p>
 +
A. Newspaper and magazine
 +
<br>
 +
B. Television and radio
 +
<br>
 +
C. Healthy classroom
 +
<br>
 +
D. The Internet
 +
<br>
 +
E. From doctor
 +
<br>
 +
F. Other
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/d/dc/BIT-PAPER22.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q23. Which important factors do you think will induce breast cancer?*(multiple choice)
 +
</p>
 +
A. Female
 +
<br>
 +
B. Over 50 years old
 +
<br>
 +
C. Immediate family member shave medical history of breast cancer
 +
<br>
 +
D. Superior living condition
 +
<br>
 +
E. Delay menopause <br>
 +
F. Fatter after menopause<br>
 +
G. Have medical history of breast cancer<br>
 +
H. Have medical history of ovarian cancer<br>
 +
I. Have atypical hyper plastic benign breast disease <br>
 +
J. Amount of alcohol <br>
 +
K. Have menstruation before 12 years old<br>
 +
L. Have hormone replacement therapy<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/a/a9/BIT-PAPER23.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q24. Which factors do you think will increase the risk of breast cancer?*(multiple choice)
 +
</p>
 +
A. Closed relative has breast cancer <br>
 +
B. Has breast cancer of other cancer<br>
 +
C. Hyperplasia of mammary glands for a long time <br>
 +
D. Endocrine disorders for a long time<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2016/9/98/BIT-PAPER24.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q25. Which sign do you think is danger for breast cancer?*(multiple choice)
 +
</p>
 +
A. Find lump in the breast<br>
 +
B. Breast hyperplasia increase happened in woman over 30 years old<br>
 +
C. Breast reduction and nipple up <br>
 +
D. Have ulcer around breast or nipple<br>
 +
E. Within the breast skin to shrink<br>
 +
F. Nipple have hemorrhagic discharge <br>
 +
G. Axillary lymph node enlargement<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/8/80/BIT-PAPER25.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q26.Do you know how to check breast by yourself?*
 +
</p>
 +
○A. YES
 +
<br>
 +
○B. No
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/e/ea/BIT-PAPER26.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q27. What is the best way to treat breast cancer in the early?*
 +
</p>
 +
A. Surgery<br>
 +
B. Radiation therapy <br>
 +
C. Chemotherapy <br>
 +
D. Endocrine therapy <br>
 +
E. Immunotherapy <br>
 +
F. Chinese medicine for therapy<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/3/3c/BIT-PAPER27.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q28. How often do you think to have a mammary gland film making check?*
 +
</p>
 +
A. Once a month<br>
 +
B. Three months <br>
 +
C. Half a year <br>
 +
D. Once a year <br>
 +
E. Two years<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/8/8d/BIT-PAPER28.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
 +
<p>
 +
Q29. Do you know about Synthetic biology?*
 +
</p>
 +
A. YES
 +
<br>
 +
B. NO
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/9/9e/BIT-PAPER29.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q30. Would you mind doing treatment in vitro with harmless microbial?*
 +
</p>
 +
A. YES <br>
 +
B. Never mind <br>
 +
C. No<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/d/d1/BIT-PAPER30.gif" class="img-responsive" alt="..."><br><br>
 +
 +
<p>
 +
Q31. How much do you think is suitable for a medical?*
 +
</p>
 +
A. Below￥500
 +
<br>
 +
B. ￥500-1000
 +
<br>
 +
C. Above￥1000<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/a/ab/BIT-PAPER31.gif" class="img-responsive" alt="..."><br><br>
 +
 
<p>
 
<p>
−
Deng Yanzi contributed to this story<br><br><br><br><br><br>
+
Q32. Which price is reasonable for our device?
 
</p>
 
</p>
 +
○A. ￥100-500 <br>
 +
○B. ￥500-1000 <br>
 +
○C. ￥1000-1500 <br>
 +
○D. Above￥1500<br><br>
 +
<img src="https://static.igem.org/mediawiki/2016/f/f6/BIT-PAPER32.gif" class="img-responsive" alt="..."><br><br>
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 17:16, 19 October 2016

<!DOCTYPE html> Biology

PRACTICE

HUMAN PRACTICES








Questionnaire

This year we issued the questionnaire through the Internet, with both Chinese and English versions and got information after collecting them. The questions were centered on the personal health, physical examination, the understanding of breast cancer and the team’s products. It was distributed online in January and ended in March and we received 299 valid feedbacks.








Survey

PARTⅠ Identity information

Q1. What is your gender?

A. Male
B. Female

...

Q2. How old are you?

A. Below20
B. 21-30
C. 31-40
D. Above41

...

Q3. What is your record of formal schooling?*

A. Junior high school
B. High school
C. College
D. Undergraduate or over it

...

Q4. Have you been married?*


A. YES
B. NO

...

Q5. What is your salary each month?*

A. ￥0
B. ￥0-3000
C. ￥3000-10000
D. Above￥10000

...

Q6. Is the field you are engaged in is connected to the biological?*

A. YES
B. NO

...

Q7. How much do you cost each month?*

A. Below￥1000
B. ￥1000-2000
C. ￥2000-3000
D. Above￥3000

...

Q8. Have you bought social security or medical insurance?*

A. YES
B. NO

...

PART Ⅱ Physical condition

Q9. What is your figure?*

A. Thin
B. Normal
C. Fat

...

Q10. What is your physical quality?*

A. Bad
B. Normal
C. Well

...

Q11. How often do you do exercise each week?*

A. Never
B. 1-2times
C. Above 3 times

...

Q12. Why do you exercise?*

A. For hobby
B. Someone organize
C. Keep fit

...

Q13. How long do you exercise each time?*

A. 10-20minutes
B. 20-30minutes
C. Above30minutes

...

Q14.Have you ever had significant medical history?*

A. YES
B. NO

...

Q15.When do you go to bed?*

A. Before10:00P.M
B. 10:00P.M-12:00P.M
C. After12:00P.M

...

Q16. Have you ever smoked or drunk?*

A. Neither
B. Smoked
C. Drunk
D. Both

...

Q17. Do you have breakfast?*

A. Never
B. Sometimes
C. Always
D. Everyday

...

PART Ⅲ About medical

Q18.How often do you do a physical examination ?*

A. Every year
B. Not regular
C. Never

...

Q19. What do you think about the price of medical?*

A. Expensive
B. Normal
C. Cheap

...

Q20. What is the meaning of medical?*

A. Predict
B. Check disease
C. Other

...

Q21. Can you understand the medical report?*

A. YES
B. NO
...

PART Ⅳ About breast cancer

Q22.Which way do you use to know about breast cancer?*(multiple choice)

A. Newspaper and magazine
B. Television and radio
C. Healthy classroom
D. The Internet
E. From doctor
F. Other

...

Q23. Which important factors do you think will induce breast cancer?*(multiple choice)

A. Female
B. Over 50 years old
C. Immediate family member shave medical history of breast cancer
D. Superior living condition
E. Delay menopause
F. Fatter after menopause
G. Have medical history of breast cancer
H. Have medical history of ovarian cancer
I. Have atypical hyper plastic benign breast disease
J. Amount of alcohol
K. Have menstruation before 12 years old
L. Have hormone replacement therapy

...

Q24. Which factors do you think will increase the risk of breast cancer?*(multiple choice)

A. Closed relative has breast cancer
B. Has breast cancer of other cancer
C. Hyperplasia of mammary glands for a long time
D. Endocrine disorders for a long time

...

Q25. Which sign do you think is danger for breast cancer?*(multiple choice)

A. Find lump in the breast
B. Breast hyperplasia increase happened in woman over 30 years old
C. Breast reduction and nipple up
D. Have ulcer around breast or nipple
E. Within the breast skin to shrink
F. Nipple have hemorrhagic discharge
G. Axillary lymph node enlargement

...

Q26.Do you know how to check breast by yourself?*

○A. YES
○B. No

...

Q27. What is the best way to treat breast cancer in the early?*

A. Surgery
B. Radiation therapy
C. Chemotherapy
D. Endocrine therapy
E. Immunotherapy
F. Chinese medicine for therapy

...

Q28. How often do you think to have a mammary gland film making check?*

A. Once a month
B. Three months
C. Half a year
D. Once a year
E. Two years

...

Q29. Do you know about Synthetic biology?*

A. YES
B. NO

...

Q30. Would you mind doing treatment in vitro with harmless microbial?*

A. YES
B. Never mind
C. No

...

Q31. How much do you think is suitable for a medical?*

A. Below￥500
B. ￥500-1000
C. Above￥1000

...

Q32. Which price is reasonable for our device?

○A. ￥100-500
○B. ￥500-1000
○C. ￥1000-1500
○D. Above￥1500

...

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